Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings.

Autor: Beulah G; Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India, 522510., Divya D; Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India, 522510., Kumar NSS; Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India, 522213., Sravya MVN; Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India, 522510., Rao KG; Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India, 522510., Chintagunta AD; Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India, 522213., Divya G; Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India, 522213., Chandana SH; Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India, 522213., Blessy BD; Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India, 522213., Simhachalam G; Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India, 522510. chalamgp99@gmail.com.
Jazyk: angličtina
Zdroj: AMB Express [AMB Express] 2021 Oct 08; Vol. 11 (1), pp. 135. Date of Electronic Publication: 2021 Oct 08.
DOI: 10.1186/s13568-021-01295-5
Abstrakt: Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an immense need for the discovery of alternative and efficient treatment for quick recovery of diseased fishes. In the present study, Suaeda maritima leaf extracts (hexane, diethyl ether, ethanol, and water) were screened for in vitro and in vivo antibacterial and antioxidant activities. Out of all the four extracts, ethanolic extract showed highest antibacterial activity against S. aureus (4.9 ± 1.3 mm), B. subtilis (1.6 ± 0.3 mm), K. pneumoniae (4.2 ± 1.8 mm), and P. aeruginosa (4.1 ± 1.2 mm). Similarly, antioxidant activity was also higher for ethanolic extract (500 µg/mL) based on DPPH radical scavenging ability (71.6 ± 1.4%) and reducing potential (149 μg/mL) assays. Further, ethanolic extract was purified consecutively via column chromatography and preparative TLC where an active fraction was selected based on highest antibacterial (10.1 ± 1.4 mm) and antioxidant properties (82.3 ± 2.8%). Active fraction was loaded onto mass spectroscopy and identified the presence of four active constituents such as 1,2,9,10-tetramethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-3-yl) methanol; 3',7-Dimethoxy-3-hydroxyflavone; Saponin and (19R)9acetyl19hydroxy10,14dimethyl20oxopentacyclo[11.8.0.0 < 2,10 > .0 < 4,9 > .0 < 14,19 >]henicos-17-yl-acetate. Besides, in vivo studies were conducted on Catla catla fingerlings infected with P. aeruginosa under laboratory conditions. The fingerlings were segregated into 5 groups, among which group 4 and 5 were treated with crude and purified extracts. Both the extracts were efficient in treating infected fingerlings and recorded 100% survival rate which is even better than group-3 treated with a synthetic antibiotic (77%). Hence, S. maritima leaf extract can be considered as a possible alternative medicine in aquaculture.
(© 2021. The Author(s).)
Databáze: MEDLINE
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