| Autor: |
Opstad IS, Hansen DH, Acuña S, Ströhl F, Priyadarshi A, Tinguely JC, Dullo FT, Dalmo RA, Seternes T, Ahluwalia BS, Agarwal K |
| Jazyk: |
angličtina |
| Zdroj: |
Optics express [Opt Express] 2021 Jul 19; Vol. 29 (15), pp. 23368-23380. |
| DOI: |
10.1364/OE.423809 |
| Abstrakt: |
Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique multiple signal classification algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2-3.6-fold compared to the corresponding conventional images. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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