Hydroxycinnamic acid-spermidine amides from Tetragonisca angustula honey as anti-Neospora caninum: In vitro and in silico studies.

Autor: Lima ÂCO; Graduate Program in Biotechnology, State University of Feira de Santana - UEFS, Feira de Santana, Brazil.; Veterinary, Biochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil., Conceição RS; Graduate Program in Biotechnology, State University of Feira de Santana - UEFS, Feira de Santana, Brazil., Freitas LS; Veterinary, Biochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil., de Carvalho CAL; Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil., Conceição ALDS; Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil., Freitas HF; Laboratory of Bioinformatics and Molecular Modeling (LaBiMM), Pharmacy College, Federal University of Bahia (UFBA), Salvador, Brazil., Pita SSDR; Laboratory of Bioinformatics and Molecular Modeling (LaBiMM), Pharmacy College, Federal University of Bahia (UFBA), Salvador, Brazil., Ifa DR; Department of Chemistry, Center for Research in Mass Spectrometry, York University, Toronto, Ontario, Canada., Pinheiro AM; Veterinary, Biochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil., Branco A; Graduate Program in Biotechnology, State University of Feira de Santana - UEFS, Feira de Santana, Brazil.; Laboratory of Phytochemistry, State University of Feira de Santana - UEFS, Feira de Santana, Brazil.
Jazyk: angličtina
Zdroj: Chemical biology & drug design [Chem Biol Drug Des] 2021 Dec; Vol. 98 (6), pp. 1104-1115. Date of Electronic Publication: 2021 Oct 17.
DOI: 10.1111/cbdd.13969
Abstrakt: Tetragonisca angustula honey was fractioned in a SiO 2 column to furnish three fractions (A-C) in which four hydroxycinnamic acid-Spermidine amides (HCAAs), known as N', N″, N‴-tris-p-coumaroyl spermidine, N', N″-dicaffeoyl, N‴-coumaroyl spermidine, N', N″, N‴-tris-caffeoyl spermidine and N', N″-dicaffeoyl and N‴-feruloyl spermidine were identified in the fractions B and C by electrospray ionization tandem mass spectrometry. A primary culture model previously infected with Neospora caninum (72 h) was used to evaluate the honey fractions (A-C) for two-time intervals: 24 and 72 h. Parasitic reduction ranged from 38% on fraction C (12.5 µg/ml), after 24 h, to 54% and 41% with fractions B and C (25 µg/ml) after 72 h of treatment, respectively. Additionally, HCAAs did not show any cell toxicity for 24 and 72 h. For infected cultures (72 h), the active fractions B (12.5 µg/ml) and C (25 µg/ml) decreased their NO content. In silico studies suggest that HCAAs may affect the parasite's redox pathway and improve the oxidative effect of NO released from infected cells. Here, we presented for the first time, that HCAAs from T. angustula honey have the potential to inhibit the growth of N. caninum protozoa.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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