| Autor: |
Takejima AL; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Francisco JC, Simeoni RB; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., de Noronha L; Pathology Department, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Garbers LAFM, Foltz KM; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Junior PABM; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Souza IC; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Pinho RA; Laboratory of Exercise Biochemistry in Health (BioEx) of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil., Carvalho KAT; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Curitiba, PR, Brazil., Guarita-Souza LC; Experimental Laboratory, The Institute of Biological and Health Sciences of the Pontifical Catholic University of Paraná (PUCPR), Curitiba, PR, Brazil. |
| Abstrakt: |
Stem cells (SC) and amniotic membrane (AM) are recognized for their beneficial impacts on the healing of cutaneous wounds. Thus, this study evaluated the capacity of tissue repair in a skin lesion rat model. Forty Wistar rats were randomized into four groups: group I - control, with full-thickness lesions on the back, without SC or AM; group II-injected SC; group III - covered by AM; group IV-injected SC and covered by AM. Lesion closure was assessed using contraction rate (Cr). Histochemical and immunohistochemical analyses were performed, and collagen, elastic fibers, fibroblast differentiation factor (TGF-β), collagen remodeling (MMP-8), and the number of myofibroblasts and blood vessels (α-SMA) were evaluated. On the 7 th postoperative day, Cr 1 st -7 th day levels were higher in groups III and IV. However, on the 28 th day, Cr 1 st -28 th day were higher in the control group. Picrosirius staining showed that type I collagen was predominant in all groups; however, the SC + AM group obtained a higher average when compared to the control group. Elastic fiber analysis showed a predominance in groups that received treatment. Groups II and IV showed the lowest expression levels of TGF-β and MMP-8, and α-SMA was significantly lower in group IV. The application of SC and AM accelerated the initial healing phase, probably owing to their anti-inflammatory effect that favored early formation of collagen and elastic fibers. |
