Dual imaging of dendritic spines and mitochondria in vivo reveals hotspots of plasticity and metabolic adaptation to stress.
Autor: | Dromard Y; Institut de Génomique Fonctionnelle, Université de Montpellier, INSERM, CNRS, 34090, Montpellier, France., Arango-Lievano M; Institut de Génomique Fonctionnelle, Université de Montpellier, INSERM, CNRS, 34090, Montpellier, France., Fontanaud P; Institut de Génomique Fonctionnelle, Université de Montpellier, INSERM, CNRS, 34090, Montpellier, France.; Imagerie du petit animal de Montpellier, 34090, Montpellier, France., Tricaud N; I-Stem, UEVE, INSERM, AFM, 91100, Corbeil-Essonnes, France., Jeanneteau F; Institut de Génomique Fonctionnelle, Université de Montpellier, INSERM, CNRS, 34090, Montpellier, France. |
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Jazyk: | angličtina |
Zdroj: | Neurobiology of stress [Neurobiol Stress] 2021 Sep 21; Vol. 15, pp. 100402. Date of Electronic Publication: 2021 Sep 21 (Print Publication: 2021). |
DOI: | 10.1016/j.ynstr.2021.100402 |
Abstrakt: | Metabolic adaptation is a critical feature of synaptic plasticity. Indeed, synaptic plasticity requires the utilization and resupply of metabolites, in particular when the turnover is high and fast such as in stress conditions. What accounts for the localized energy burden of the post-synaptic compartment to the build up of chronic stress is currently not understood. We used in vivo microscopy of genetically encoded fluorescent probes to track changes of mitochondria, dendritic spines, ATP and H2O2 levels in pyramidal neurons of cortex before and after chronic unpredictable mild stress. Data revealed hotspots of postsynaptic mitochondria and dendritic spine turnover. Pharmacogenetic approach to force expression of the metabolic stress gene NR4A1 caused the fragmentation of postsynaptic mitochondria and loss of proximal dendritic spine clusters, whereas a dominant-negative mutant counteracted the effect of chronic stress. When fragmented, dendritic mitochondria produced lesser ATP at resting state and more on acute demand. This corresponded with significant production of mitochondrial H2O2 oxidative species in the dendritic compartment. Together, data indicate that pyramidal neurons adjust proximal dendritic spine turnover and mitochondria functions in keeping with synaptic demands. Competing Interests: No authors are declaring a conflict of interest. (© 2021 The Authors.) |
Databáze: | MEDLINE |
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