Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

Autor:	D'Gama PP; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgsons Gate 1, 7491 Trondheim, Norway; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Qiu T; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore., Cosacak MI; German Center for Neurodegenerative Diseases (DZNE), Helmholtz Association, Tatzberg 41, 01307 Dresden, Germany., Rayamajhi D; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore., Konac A; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Hansen JN; Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Ringers C; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgsons Gate 1, 7491 Trondheim, Norway; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Acuña-Hinrichsen F; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Hui SP; S. N. Pradhan Centre for Neurosciences, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India., Olstad EW; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Chong YL; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore., Lim CKA; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore., Gupta A; Resolve Biosciences GmbH, Creative Campus Monheim, Gebäude A03, Alfred-Nobel-Str.10, 40789 Monheim am Rhein, Germany., Ng CP; A(∗)STAR Microscopy Platform, Research Support Center, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore., Nilges BS; Resolve Biosciences GmbH, Creative Campus Monheim, Gebäude A03, Alfred-Nobel-Str.10, 40789 Monheim am Rhein, Germany., Kashikar ND; Resolve Biosciences GmbH, Creative Campus Monheim, Gebäude A03, Alfred-Nobel-Str.10, 40789 Monheim am Rhein, Germany., Wachten D; Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Liebl D; A(∗)STAR Microscopy Platform, Research Support Center, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore., Kikuchi K; Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka 564-8565, Japan., Kizil C; German Center for Neurodegenerative Diseases (DZNE), Helmholtz Association, Tatzberg 41, 01307 Dresden, Germany; Department of Neurology and The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, 650 W 168th St, New York, NY 10032, USA., Yaksi E; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway., Roy S; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore; Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, Singapore 119288, Singapore. Electronic address: sudipto@imcb.a-star.edu.sg., Jurisch-Yaksi N; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgsons Gate 1, 7491 Trondheim, Norway; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway. Electronic address: nathalie.jurisch-yaksi@ntnu.no.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2021 Oct 05; Vol. 37 (1), pp. 109775.
DOI:	10.1016/j.celrep.2021.109775
Abstrakt:	Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monociliated cells and generate directional flow patterns. These ciliated cells have different developmental origins and are genetically heterogenous with respect to expression of the Foxj1 family of ciliary master regulators. Finally, we show that cilia loss from the tela choroida and choroid plexus or global perturbation of multiciliation does not affect overall brain or spine morphogenesis but results in enlarged ventricles. Our findings establish that motile ciliated cells are generated by complementary and sequential transcriptional programs to support ventricular development. Competing Interests: Declaration of interests N.K., A.G., and B.S.N. are full-time employees of Resolve Biosciences GmbH. (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu