ROS-sensitive micelles for controlled delivery of antibiotics to combat intracellular Staphylococcus aureus -associated infections.

Autor: Qiao J; Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Southeast University, Nanjing, 211189, China. mpxiong@uga.edu.; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, 30602-2352, USA., Cui S; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, 30602-2352, USA., Xiong MP; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, 30602-2352, USA.
Jazyk: angličtina
Zdroj: Journal of materials chemistry. B [J Mater Chem B] 2021 Nov 10; Vol. 9 (43), pp. 8951-8961. Date of Electronic Publication: 2021 Nov 10.
DOI: 10.1039/d1tb01702k
Abstrakt: Bacteria can evade the immune system once they are engulfed by phagocytic host cells. This protects them against the bactericidal action of antibiotics and allows the infection to remain latent or to recur. Reactive oxygen species (ROS)-related stress has been implicated in various pathological conditions such as inflammatory diseases involving infections of host cells and can serve as a useful trigger for intracellular controlled drug delivery. We herein report on a fluorescent ROS-sensitive intracellular antibiotic delivery nanoparticle for encapsulation of rifampin (RIF) based on the principles of Förster Resonance Energy Transfer (FRET) that is capable of ratiometrically sensing H 2 O 2 levels and monitoring the drug release process. The fluorescent micelles (MFs) are formed through the self-assembly of amphiphilic diblock copolymers consisting of a poly(ethylene glycol) (PEG) segment and a fluorescent oxidation-responsive hydrophobic phenylboronic pinacol ester (PBA) block. Specifically, MFs could encapsulate the model antibiotic RIF (MF/RIF) and ROS-triggered controlled release of RIF within infected macrophages (where ROS levels are elevated) improved the elimination of intracellular bacteria compared to MF or RIF alone. This antibiotic delivery system may be especially effective at fighting intracellular pathogens that have managed to evade the immune system and could minimize exposure of normal cells and tissues to high drug concentrations.
Databáze: MEDLINE
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