Anxiety and depression-like behaviours are more frequent in aged male mice conceived by ART compared with natural conception.

Autor: Qin NX; Department of Assisted Reproductive Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China., Zhao YR; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Shi WH; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Zhou ZY; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Zou KX; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Yu CJ; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Liu X; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Dong ZH; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Mao YT; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Zhou CL; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Yu JL; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Liu XM; Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China., Sheng JZ; Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Ding GL; Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China., Zhao WL; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China., Wu YT; Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China., Huang HF; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.; Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.; The Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of Education, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Jazyk: angličtina
Zdroj: Reproduction (Cambridge, England) [Reproduction] 2021 Oct 28; Vol. 162 (6), pp. 437-448. Date of Electronic Publication: 2021 Oct 28.
DOI: 10.1530/REP-21-0175
Abstrakt: The number of children born after assisted reproductive technology (ART) is accumulating rapidly, and the health problems of the children are extensively concerned. This study aims to evaluate whether ART procedures alter behaviours in male offspring. Mouse models were utilized to establish three groups of offspring conceived by natural conception (NC), in vitro fertilization and embryo transfer (IVF-ET), and frozen-thawed embryo transfer (IVF-FET), respectively. A battery of behaviour experiments for evaluating anxiety and depression levels, including the open field test (OFT), elevated plus maze (EPM) test, light/dark transition test (L/DTT), tail suspension test (TST), forced swimming test (FST), and sucrose preference test (SPT) was carried out. Aged (18 months old), but not young (3 months old), male offspring in the IVF-ET and IVF-FET groups, compared with those in the NC group, exhibited increased anxiety and depression-like behaviours. The protein expression levels of three neurotrophins in PFC or hippocampus in aged male offspring from the IVF-ET and IVF-FET groups reduced at different extent, in comparison to NC group. RNA sequencing (RNA-Seq) was performed in the hippocampus of 18 months old offspring to further explore the gene expression profile changes in the three groups. KEGG analyses revealed the coexisted pathways, such as PI3K-Akt signalling pathway, which potentially reflected the similarity and divergence in anxiety and depression between the offspring conceived by IVF-ET and IVF-FET. Our research suggested the adverse effects of advanced age on the psychological health of children born after ART should be highlighted in the future.
Databáze: MEDLINE