High-Quality de novo Chromosome-Level Genome Assembly of a Single Bombyx mori With BmNPV Resistance by a Combination of PacBio Long-Read Sequencing, Illumina Short-Read Sequencing, and Hi-C Sequencing.

Autor:	Tang M; School of Life Sciences, Jiangsu University, Zhenjiang, China., He S; School of Life Sciences, Jiangsu University, Zhenjiang, China., Gong X; Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China.; Department of Medical Rheumatology, Columbia University, New York, NY, United States., Lü P; School of Life Sciences, Jiangsu University, Zhenjiang, China., Taha RH; Department of Sericulture, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt., Chen K; School of Life Sciences, Jiangsu University, Zhenjiang, China.
Jazyk:	angličtina
Zdroj:	Frontiers in genetics [Front Genet] 2021 Sep 16; Vol. 12, pp. 718266. Date of Electronic Publication: 2021 Sep 16 (Print Publication: 2021).
DOI:	10.3389/fgene.2021.718266
Abstrakt:	The reference genomes of Bombyx mori ( B. mori ), Silkworm Knowledge-based database (SilkDB) and SilkBase, have served as the gold standard for nearly two decades. Their use has fundamentally shaped model organisms and accelerated relevant studies on lepidoptera. However, the current reference genomes of B. mori do not accurately represent the full set of genes for any single strain. As new genome-wide sequencing technologies have emerged and the cost of high-throughput sequencing technology has fallen, it is now possible for standard laboratories to perform full-genome assembly for specific strains. Here we present a high-quality de novo chromosome-level genome assembly of a single B. mori with nuclear polyhedrosis virus ( BmNPV ) resistance through the integration of PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C sequencing. In addition, regular bioinformatics analyses, such as gene family, phylogenetic, and divergence analyses, were performed. The sample was from our unique B. mori species (NB), which has strong inborn resistance to BmNPV . Our genome assembly showed good collinearity with SilkDB and SilkBase and particular regions. To the best of our knowledge, this is the first genome assembly with BmNPV resistance, which should be a more accurate insect model for resistance studies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Tang, He, Gong, Lü, Taha and Chen.)
Databáze:	MEDLINE
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