Metabolic fasting stress is ameliorated in Kinin B1 receptor-deficient mice.

Autor: Freitas-Lima LC; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil., Budu A; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil. Electronic address: alexandre.budu@unifesp.br., Estrela GR; Department of Medicine, Discipline of Nephrology, Federal University of São Paulo, São Paulo, Brazil; Department of Clinical and Experimental Oncology, Discipline of Hematology and Hematotherapy, Federal University of São Paulo, 04037002 São Paulo, Brazil. Electronic address: g.estrela@unifesp.br., Alves-Silva T; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil. Electronic address: thais.silva13@unifesp.br., Perilhão MS; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil; Department of Biochemistry, Discipline of Molecular Biology, Federal University of São Paulo, São Paulo, Brazil; Department of Clinical and Experimental Oncology, Discipline of Hematology and Hematotherapy, Federal University of São Paulo, 04037002 São Paulo, Brazil; Department of Physical Education, Santo Amaro University (UNISA), São Paulo, Brazil. Electronic address: mperilhao@unifesp.br., Arruda AC; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil; Department of Medicine, Discipline of Nephrology, Federal University of São Paulo, São Paulo, Brazil. Electronic address: adriano_arruda@hotmail.com., Carvalho Araujo R; Department of Biophysics, Federal University of São Paulo, 04039032 São Paulo, Brazil; Department of Medicine, Discipline of Nephrology, Federal University of São Paulo, São Paulo, Brazil. Electronic address: araujo.ronaldo@unifesp.br.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2022 Apr 01; Vol. 294, pp. 120007. Date of Electronic Publication: 2021 Sep 30.
DOI: 10.1016/j.lfs.2021.120007
Abstrakt: The liver has an essential role in responding to metabolic demands under stress conditions. The organ stores, releases, and recycles metabolism-related substrates. However, it is not clear how the Kallikrein-Kinin System modulates metabolic flexibility shift between energetic sources.
Aims: To analyze the hepatic metabolism in kinin B1 receptor deficient mice (B1KO mice) under fasting conditions.
Main Methods: WT and B1KO male mice were allocated in a calorimetric cage for 7 days and 48 h before the euthanasia, half of the animals of both groups were under fasting conditions. Biochemical parameters, ketone bodies (KB), and gene expression involving the liver energetic metabolism genes were evaluated.
Key Findings: Kinin B1 receptor (B1R) modulates the metabolic shift under fasting conditions, reducing the VO 2 expenditure. A preference for carbohydrates as an energetic source is suggested, as the B1KO group did not display an increase in KB in the serum. Moreover, the B1KO animals displayed higher serum triglycerides concentration compared to WT fasting mice. Interestingly, the lack of B1R induces the increase expression of enzymes from the glycolysis and lipolysis pathways under the fed. However, under fasting, the enzymatic expression of gluconeogenesis, glyceroneogenesis, and ketogenesis of these pathways does not occur, suggesting an absence of the shift metabolism responsivity, and this condition is modulated by PDK4 under FOXO1 control.
Significance: B1R has an important role in the hepatic glucose metabolism, which in turn influences the energetic metabolism, and in long-term outcomes, such as in the decrease in hepatic glycogen stores and in the enhancement of hepatic metabolism.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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