miR-17-5p drives G2/M-phase accumulation by directly targeting CCNG2 and is related to recurrence of head and neck squamous cell carcinoma.

Autor: Huang Q; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Shen YJ; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Hsueh CY; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Guo Y; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Zhang YF; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Li JY; Department of Pediatric, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200092, China., Zhou L; Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China. zhoulent@126.com.
Jazyk: angličtina
Zdroj: BMC cancer [BMC Cancer] 2021 Oct 02; Vol. 21 (1), pp. 1074. Date of Electronic Publication: 2021 Oct 02.
DOI: 10.1186/s12885-021-08812-6
Abstrakt: Background: The human miR-17-92 polycistron is the first reported and most well-studied onco-miRNA with a cluster of seven miRNAs. miR-17-5p, a member of the miR-17-92 family, plays an important role in tumor cell proliferation, apoptosis, migration and invasion. However, few studies have shown the role of miR-17-5p in the cell cycle of head and neck squamous cell carcinoma (HNSCC).
Methods: RT-qPCR was used to detect miR-17-5p expression levels in 64 HNSCC tissues and 5 cell lines. The relationship between the expression of miR-17-5p in the tissues and the clinical characteristics of the patients was analyzed. HNSCC cells were transfected with an miR-17-5p mimic or inhibitor to evaluate cell cycle distribution by flow cytometry. Cell cycle distribution of cells transfected with target gene was evaluated using flow cytometry. Dual-luciferase reporter assay was used to detect the regulatory effect of miR-17-5p on target gene expression.
Results: In the present study, we found that miR-17-5p expression in HNSCC tissues and cell lines was remarkably increased, and miR-17-5p is related to recurrence in HNSCC patients. Silencing miR-17-5p blocked HNSCC cells in G2/M phase, whereas its overexpression propelled cell cycle progression. More importantly, we verified that miR-17-5p negatively regulated CCNG2 mRNA and protein expression by directly targeting its 3'UTR.
Conclusion: These findings suggest that miR-17-5p might act as a tumor promoter and prognostic factor for recurrence in HNSCC patients.
(© 2021. The Author(s).)
Databáze: MEDLINE
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