Protein Catalyzed Capture (PCC) Agents for Antigen Targeting.
| Autor: | Idso MN; Institute for Systems Biology, Seattle, WA, USA., Lai BT; Indi Molecular, Inc., Culver City, CA, USA., Agnew HD; Indi Molecular, Inc., Culver City, CA, USA., Heath JR; Institute for Systems Biology, Seattle, WA, USA. jim.heath@isbscience.org.; Department of Bioengineering, University of Washington, Seattle, WA, USA. jim.heath@isbscience.org. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2371, pp. 177-191. |
| DOI: | 10.1007/978-1-0716-1689-5_10 |
| Abstrakt: | The protein catalyzed capture agent (PCC) method is a powerful combinatorial screening strategy for discovering synthetic macrocyclic peptide ligands, called PCCs, to designated protein epitopes. The foundational concept of the PCC method is the use of in situ click chemistry to survey large combinatorial libraries of peptides for ligands to designated biological targets. State-of-the-art PCC screens integrate synthetic libraries of constrained macrocyclic peptides with epitope-specific targeting strategies to identify high-affinity (<100 nM) binders de novo. Automated instrumentation can accelerate PCC discovery to a rapid 2-week timeframe. Here, we describe methods to perform combinatorial screens that yield epitope-targeted PCCs. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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