2-Alkyl-4-quinolone quorum sensing molecules are biomarkers for culture-independent Pseudomonas aeruginosa burden in adults with cystic fibrosis.

Autor: Zain NMM; Institute of Pharmaceutical Science, King's College London, London, UK.; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK., Webb K; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK.; Division of Epidemiology and Public Health, University of Nottingham, City Hospital Campus, Nottingham, UK., Stewart I; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK.; National Heart and Lung Institute, Imperial College London, London, UK., Halliday N; National Biofilms Innovation Centre, University of Nottingham Biodiscovery Institute, School of Life Sciences, University of Nottingham, Nottingham, UK., Barrett DA; Centre for Analytical Bioscience, Division of Advanced Materials and Healthcare Technologies, School of Pharmacy, University of Nottingham, Nottingham, UK., Nash EF; West Midlands Adult CF Centre, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Whitehouse JL; West Midlands Adult CF Centre, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Honeybourne D; West Midlands Adult CF Centre, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Smyth AR; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK.; Division of Child Health, Obstetrics and Gynaecology, University of Nottingham, Nottingham, UK., Forrester DL; University of Queensland, Northside Clinical Unit, Brisbane, Queensland, Australia.; Thoracic Programme, The Prince Charles Hospital, Brisbane, Australia., Knox AJ; Division of Respiratory Medicine, University of Nottingham, City Hospital Campus, Nottingham, UK.; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK., Williams P; National Biofilms Innovation Centre, University of Nottingham Biodiscovery Institute, School of Life Sciences, University of Nottingham, Nottingham, UK., Fogarty A; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK.; Division of Epidemiology and Public Health, University of Nottingham, City Hospital Campus, Nottingham, UK., Cámara M; National Biofilms Innovation Centre, University of Nottingham Biodiscovery Institute, School of Life Sciences, University of Nottingham, Nottingham, UK., Bruce KD; Institute of Pharmaceutical Science, King's College London, London, UK.; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK., Barr HL; Wolfson Cystic Fibrosis Centre, Department of Respiratory Medicine, Nottingham University Hospitals NHS Trust, Nottingham, UK.; Nottingham NIHR Biomedical Research Centre, Nottingham MRC Molecular Pathology Node, Nottingham, UK.
Jazyk: angličtina
Zdroj: Journal of medical microbiology [J Med Microbiol] 2021 Oct; Vol. 70 (10).
DOI: 10.1099/jmm.0.001420
Abstrakt: Introduction. Pseudomonas aeruginosa produces quorum sensing signalling molecules including 2-alkyl-4-quinolones (AQs), which regulate virulence factor production in the cystic fibrosis (CF) airways. Hypothesis/Gap statement. Culture can lead to condition-dependent artefacts which may limit the potential insights and applications of AQs as minimally-invasive biomarkers of bacterial load. Aim. We aimed to use culture-independent methods to explore the correlations between AQ levels and live P. aeruginosa load in adults with CF. Methodology. Seventy-five sputum samples at clinical stability and 48 paired sputum samples obtained at the beginning and end of IV antibiotics for a pulmonary exacerbation in adults with CF were processed using a viable cell separation technique followed by quantitative P. aeruginosa polymerase chain reaction (qPCR). Live P. aeruginosa qPCR load was compared with the concentrations of three AQs (HHQ, NHQ and HQNO) detected in sputum, plasma and urine. Results. At clinical stability and the beginning of IV antibiotics for pulmonary exacerbation, HHQ, NHQ and HQNO measured in sputum, plasma and urine were consistently positively correlated with live P. aeruginosa qPCR load in sputum, compared to culture. Following systemic antibiotics live P. aeruginosa qPCR load decreased significantly ( P <0.001) and was correlated with a reduction in plasma NHQ (plasma: r=0.463, P =0.003). Conclusion. In adults with CF, AQ concentrations correlated more strongly with live P. aeruginosa bacterial load measured by qPCR compared to traditional culture. Prospective studies are required to assess the potential of systemic AQs as biomarkers of P. aeruginosa bacterial burden.
Databáze: MEDLINE