Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS.
| Autor: | Brandenburg F; Institute of Plant Biochemistry, Heinrich Heine University, Duesseldorf, Germany., Schoffman H; Department of Plant and Environmental Sciences, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel., Keren N; Department of Plant and Environmental Sciences, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel., Eisenhut M; Institute of Plant Biochemistry, Heinrich Heine University, Duesseldorf, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2017 Dec 05; Vol. 7 (23), pp. e2623. Date of Electronic Publication: 2017 Dec 05 (Print Publication: 2017). |
| DOI: | 10.21769/BioProtoc.2623 |
| Abstrakt: | Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 ( Synechocystis ), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen ( Keren et al. , 2002 ). In this protocol, we describe a method to quantify the periplasmic and intracellular pools of Mn in Synechocystis accurately, using inductively coupled plasma mass spectrometry (ICP-MS). (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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