The genome of the zoonotic malaria parasite Plasmodium simium reveals adaptations to host switching.

Autor: Mourier T; Pathogen Genomics Laboratory, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., de Alvarenga DAM; Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, MG, 30190-009, Brazil., Kaushik A; Pathogen Genomics Laboratory, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., de Pina-Costa A; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Pesquisa Clínica em Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis, RJ, 25964-004, Brazil., Douvropoulou O; Pathogen Genomics Laboratory, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Guan Q; Pathogen Genomics Laboratory, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Guzmán-Vega FJ; Computational Bioscience Research Center, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Forrester S; Department of Biology and York Biomedical Research Institute, University of York, Wentworth Way, York, YO10 5DD, UK., de Abreu FVS; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz (IOC), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil., Júnior CB; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil., de Souza Junior JC; Universidade Regional de Blumenau (FURB), Centro de Pesquisas Biológicas de Indaial (CEPESBI)/ Projeto bugio, Blumenau, Indaial, SC, Brazil., Moreira SB; Centro de Primatologia do Rio de Janeiro (CPRJ/Inea), Guapimirim, RJ, 25940-000, Brazil., Hirano ZMB; Universidade Regional de Blumenau (FURB), Centro de Pesquisas Biológicas de Indaial (CEPESBI)/ Projeto bugio, Blumenau, Indaial, SC, Brazil., Pissinatti A; Centro de Primatologia do Rio de Janeiro (CPRJ/Inea), Guapimirim, RJ, 25940-000, Brazil., Ferreira-da-Cruz MF; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil., de Oliveira RL; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz (IOC), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil., Arold ST; Computational Bioscience Research Center, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.; Centre de Biologie Structurale, CNRS, INSERM, Université de Montpellier, 34090, Montpellier, France., Jeffares DC; Department of Biology and York Biomedical Research Institute, University of York, Wentworth Way, York, YO10 5DD, UK., Brasil P; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil.; Laboratório de Pesquisa Clínica em Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil., de Brito CFA; Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, MG, 30190-009, Brazil., Culleton R; Division of Molecular Parasitology, Proteo-Science Center, Ehime University, Toon, Ehime, 791-0295, Japan., Daniel-Ribeiro CT; Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil. malaria@fiocruz.br.; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, 21040-360, Brazil. malaria@fiocruz.br., Pain A; Pathogen Genomics Laboratory, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia. arnab.pain@kaust.edu.sa.; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20 W10 Kita-ku, Sapporo, Japan. arnab.pain@kaust.edu.sa.
Jazyk: angličtina
Zdroj: BMC biology [BMC Biol] 2021 Oct 01; Vol. 19 (1), pp. 219. Date of Electronic Publication: 2021 Oct 01.
DOI: 10.1186/s12915-021-01139-5
Abstrakt: Background: Plasmodium simium, a malaria parasite of non-human primates (NHP), was recently shown to cause zoonotic infections in humans in Brazil. We sequenced the P. simium genome to investigate its evolutionary history and to identify any genetic adaptions that may underlie the ability of this parasite to switch between host species.
Results: Phylogenetic analyses based on whole genome sequences of P. simium from humans and NHPs reveals that P. simium is monophyletic within the broader diversity of South American Plasmodium vivax, suggesting P. simium first infected NHPs as a result of a host switch of P. vivax from humans. The P. simium isolates show the closest relationship to Mexican P. vivax isolates. Analysis of erythrocyte invasion genes reveals differences between P. vivax and P. simium, including large deletions in the Duffy-binding protein 1 (DBP1) and reticulocyte-binding protein 2a genes of P. simium. Analysis of P. simium isolated from NHPs and humans revealed a deletion of 38 amino acids in DBP1 present in all human-derived isolates, whereas NHP isolates were multi-allelic.
Conclusions: Analysis of the P. simium genome confirmed a close phylogenetic relationship between P. simium and P. vivax, and suggests a very recent American origin for P. simium. The presence of the DBP1 deletion in all human-derived isolates tested suggests that this deletion, in combination with other genetic changes in P. simium, may facilitate the invasion of human red blood cells and may explain, at least in part, the basis of the recent zoonotic infections.
(© 2021. The Author(s).)
Databáze: MEDLINE
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