Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat's brain in vitro .
| Autor: | Oyeniran OH; Department of Biochemistry, Federal University Oye - Ekiti P.M.B. 373, Ekiti State, Nigeria.; Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Unit, Federal University of Technology, Akure, Nigeria., Ademiluyi AO; Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Unit, Federal University of Technology, Akure, Nigeria., Oboh G; Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Unit, Federal University of Technology, Akure, Nigeria. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of basic and clinical physiology and pharmacology [J Basic Clin Physiol Pharmacol] 2020 Dec 10; Vol. 32 (5), pp. 987-994. Date of Electronic Publication: 2020 Dec 10. |
| DOI: | 10.1515/jbcpp-2020-0144 |
| Abstrakt: | Objectives: Rauvolfia vomitoria is a medicinal plant used traditionally in Africa in the management of several human diseases including psychosis. However, there is inadequate scientific information on the potency of the phenolic constituents of R. vomitoria leaf in the management of neurodegeneration. Therefore, this study characterized the phenolic constituents and investigated the effects of aqueous and methanolic extracts of R. vomitoria leaf on free radicals, Fe 2+ -induced lipid peroxidation, and critical enzymes linked to neurodegeneration in rat's brain in vitro . Methods: The polyphenols were evaluated by characterizing phenolic constituents using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). The antioxidant properties were assessed through the extracts ability to reduce Fe 3+ to Fe 2+ ; inhibit ABTS, DPPH, and OH radicals and Fe 2+ -induced lipid peroxidation. The effects of the extracts on AChE and MAO were also evaluated. Results: The phenolic characterization of R. vomitoria leaf revealed that there were more flavonoids present. Both aqueous and methanolic extracts of R. vomitoria leaf had inhibitory effects with the methanolic extract having higher significant (p≤0.05) free radicals scavenging ability coupled with inhibition of monoamine oxidases. However, there was no significant (p≤0.05) difference obtained in the inhibition of lipid peroxidation and cholinesterases. Conclusion: This study suggests that the rich phenolic constituents of R. vomitoria leaf might contribute to the observed antioxidative and neuroprotective effects. The methanolic extract was more potent than the aqueous extract; therefore, extraction of R. vomitoria leaf with methanol could offer better health-promoting effects in neurodegenerative condition. (© 2020 Walter de Gruyter GmbH, Berlin/Boston.) |
| Databáze: | MEDLINE |
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