Urinary Metabolites as Predictors of Acute Mountain Sickness Severity.

Autor:	Sibomana I; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States.; Air Force Research Laboratory, 711th Human Performance Wing, Wright-Patterson AFB, Dayton, OH, United States., Foose DP; Department of Computer Science and Engineering, Wright State University, Dayton, OH, United States., Raymer ML; Department of Computer Science and Engineering, Wright State University, Dayton, OH, United States., Reo NV; Boonshoft School of Medicine, Wright State University, Dayton, OH, United States., Karl JP; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA, United States., Berryman CE; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA, United States.; Department of Nutrition, Food, and Exercise Sciences, Florida State University, Tallahassee, FL, United States.; Oak Ridge Institute of Science and Education, Belcamp, MD, United States., Young AJ; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States.; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA, United States.; Oak Ridge Institute of Science and Education, Belcamp, MD, United States., Pasiakos SM; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA, United States., Mauzy CA; Air Force Research Laboratory, 711th Human Performance Wing, Wright-Patterson AFB, Dayton, OH, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2021 Sep 13; Vol. 12, pp. 709804. Date of Electronic Publication: 2021 Sep 13 (Print Publication: 2021).
DOI:	10.3389/fphys.2021.709804
Abstrakt:	Individuals sojourning at high altitude (≥2,500m) often develop acute mountain sickness (AMS). However, substantial unexplained inter-individual variability in AMS severity exists. Untargeted metabolomics assays are increasingly used to identify novel biomarkers of susceptibility to illness, and to elucidate biological pathways linking environmental exposures to health outcomes. This study used untargeted nuclear magnetic resonance (NMR)-based metabolomics to identify urine metabolites associated with AMS severity during high altitude sojourn. Following a 21-day stay at sea level (SL; 55m), 17 healthy males were transported to high altitude (HA; 4,300m) for a 22-day sojourn. AMS symptoms measured twice daily during the first 5days at HA were used to dichotomize participants according to AMS severity: moderate/severe AMS (AMS; n =11) or no/mild AMS (NoAMS; n =6). Urine samples collected on SL day 12 and HA days 1 and 18 were analyzed using proton NMR tools and the data were subjected to multivariate analyses. The SL urinary metabolite profiles were significantly different ( p ≤0.05) between AMS vs. NoAMS individuals prior to high altitude exposure. Differentially expressed metabolites included elevated levels of creatine and acetylcarnitine, and decreased levels of hypoxanthine and taurine in the AMS vs. NoAMS group. In addition, the levels of two amino acid derivatives (4-hydroxyphenylpyruvate and N-methylhistidine) and two unidentified metabolites (doublet peaks at 3.33ppm and a singlet at 8.20ppm) were significantly different between groups at SL. By HA day 18, the differences in urinary metabolites between AMS and NoAMS participants had largely resolved. Pathway analysis of these differentially expressed metabolites indicated that they directly or indirectly play a role in energy metabolism. These observations suggest that alterations in energy metabolism before high altitude exposure may contribute to AMS susceptibility at altitude. If validated in larger cohorts, these markers could inform development of a non-invasive assay to screen individuals for AMS susceptibility prior to high altitude sojourn. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Sibomana, Foose, Raymer, Reo, Karl, Berryman, Young, Pasiakos and Mauzy.)
Databáze:	MEDLINE
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