Silver nanoparticles (AgNPs) internalization and passage through the Lactuca sativa (Asteraceae) outer cell wall.

Autor: de Paiva Pinheiro SK; Biomaterials Laboratory (BIOMAT), Department of Metallurgical Engineering and Materials (DEMM) and Analytical Center, Federal University of Ceará (UFC), Campus do Pici Fortaleza, CEP 60455-900, Fortaleza, CE, Brazil., Rangel Miguel TBA; Biotechnology Laboratory, Food Engineering Department, Federal University of Ceará (UFC), Campus do Pici Fortaleza, Fortaleza, CE, Brazil., Chaves MM; Biomaterials Laboratory (BIOMAT), Department of Metallurgical Engineering and Materials (DEMM) and Analytical Center, Federal University of Ceará (UFC), Campus do Pici Fortaleza, CEP 60455-900, Fortaleza, CE, Brazil., Barros FCF; Advanced Functional Materials Laboratory (LaMFA), Department of Physics, Federal University of Ceará, Fortaleza, CE, Brazil., Farias CP; Advanced Functional Materials Laboratory (LaMFA), Department of Physics, Federal University of Ceará, Fortaleza, CE, Brazil., de Moura TA; Tip Enhanced Raman Spectroscopy Laboratory, Department of Physics, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Ferreira OP; Advanced Functional Materials Laboratory (LaMFA), Department of Physics, Federal University of Ceará, Fortaleza, CE, Brazil., Paschoal AR; Tip Enhanced Raman Spectroscopy Laboratory, Department of Physics, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Souza Filho AG; Physics Department, School of Science, Federal University of Ceará (UFC), Campus do Pici Fortaleza, Fortaleza 60455-900, CE, Brazil., de Castro Miguel E; Biomaterials Laboratory (BIOMAT), Department of Metallurgical Engineering and Materials (DEMM) and Analytical Center, Federal University of Ceará (UFC), Campus do Pici Fortaleza, CEP 60455-900, Fortaleza, CE, Brazil.
Jazyk: angličtina
Zdroj: Functional plant biology : FPB [Funct Plant Biol] 2021 Oct; Vol. 48 (11), pp. 1113-1123.
DOI: 10.1071/FP21161
Abstrakt: Silver nanoparticle (AgNPs) toxicity is related to nanoparticle interaction with the cell wall of microorganisms and plants. This interaction alters cell wall conformation with increased reactive oxygen species (ROS) in the cell. With the increase of ROS in the cell, the dissolution of zero silver (Ag0) to ionic silver (Ag+) occurs, which is a strong oxidant agent to the cellular wall. AgNP interaction was evaluated by transmission electron microscopy (TEM) on Lactuca sativa roots, and the mechanism of passage through the outer cell wall (OCW) was also proposed. The results suggest that Ag+ binds to the hydroxyls (OH) present in the cellulose structure, thus causing the breakdown of the hydrogen bonds. Changes in cell wall structure facilitate the passage of AgNPs, reaching the plasma membrane. According to the literature, silver nanoparticles with an average diameter of 15nm are transported across the membrane into the cells by caveolines. This work describes the interaction between AgNPs and the cell wall and proposes a transport model through the outer cell wall.
Databáze: MEDLINE
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