Highly differential count of circulating and tumor infiltrating immune cells in patients with non-HCV/non-HBV hepatocellular carcinoma.

Autor: Schoenberg MB; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany.; German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany., Zhu T; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Hao J; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Bucher JN; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Li X; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Li X; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Han Y; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Koliogiannis D; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Svihla M; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany., Guba MO; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany.; Transplantation Center Munich, Hospital of the LMU, Campus Grosshadern, Munich, Germany., Werner J; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany.; German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany.; Bavarian Cancer Research Center (BZKF), Munich, Germany., Bazhin AV; Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377, Munich, Germany. alexandr.bazhin@med.uni-muenchen.de.; German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany. alexandr.bazhin@med.uni-muenchen.de.; Bavarian Cancer Research Center (BZKF), Munich, Germany. alexandr.bazhin@med.uni-muenchen.de.
Jazyk: angličtina
Zdroj: Cancer immunology, immunotherapy : CII [Cancer Immunol Immunother] 2022 May; Vol. 71 (5), pp. 1103-1113. Date of Electronic Publication: 2021 Sep 28.
DOI: 10.1007/s00262-021-03061-9
Abstrakt: Background: Liver transplantation and liver resection are curative options for early hepatocellular carcinoma (HCC). The outcome is in part depended on the immunological response to the malignancy. In this study, we aimed to identify immunological profiles of non-HCV/non-HBV HCC patients.
Methods: Thirty-nine immune cell subsets were measured with multicolor flow cytometry. This immunophenotyping was performed in peripheral blood (PB) and tumor specimens of 10 HCC resection patients and 10 healthy donors. The signatures of the highly differential leukocyte count (hDIF) were analyzed using multidimensional techniques. Functional capability was measured using intracellular IFN-γ staining (Trial Registration DRKS00013567).
Results: The hDIF showed activation (subsets of T-, B-, NK- and dendritic cells) and suppression (subsets of myeloid-derived suppressor cells and T- and B-regulatory cells) of the antitumor response. Principal component analysis of PB and tumor infiltrating leukocytes (TIL) illustrated an antitumor activating gradient. TILs showed functional capability by secreting IFN-γ but did not kill HCC cells.
Conclusions: In conclusion, the measurement of the hDIF shows distinct differences in immune reactions against non-HBV/non-HCV HCC and illustrates an immunosuppressive gradient toward peripheral blood.
Trial Registration: DRKS00013567.
(© 2021. The Author(s).)
Databáze: MEDLINE
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