Nyquist-exceeding high voxel rate acquisition in mesoscopic multiphoton microscopy for full-field submicron resolution resolvability.

Autor:	Borah BJ; Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan., Lee JC; Department of Life Science, National Taiwan University, Taipei 10617, Taiwan., Chi HH; Department of Life Science, National Taiwan University, Taipei 10617, Taiwan., Hsiao YT; Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan., Yen CT; Department of Life Science, National Taiwan University, Taipei 10617, Taiwan., Sun CK; Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.; Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 Aug 27; Vol. 24 (9), pp. 103041. Date of Electronic Publication: 2021 Aug 27 (Print Publication: 2021).
DOI:	10.1016/j.isci.2021.103041
Abstrakt:	The Nyquist-Shannon criterion has never been realized in a laser-scanning mesoscopic multiphoton microscope (MPM) with a large field-of-view (FOV)-resolution ratio, especially when employing a high-frequency resonant-raster-scanning. With a high optical resolution nature, a current mesoscopic-MPM either neglects the criterion and degrades the digital resolution to twice the pixel size, or reduces the FOV and/or the raster-scanning speed to avoid aliasing . We introduce a Nyquist figure-of-merit (NFOM) parameter to characterize a laser-scanning MPM in terms of its optical-resolution retrieving ability. Based on NFOM, we define the maximum aliasing-free FOV, and subsequently, a cross-over excitation wavelength, below which the FOV becomes NFOM-constrained irrespective of an optimized optical design. We validate our idea in a custom-built mesoscopic-MPM with millimeter-scale FOV yielding an ultra-high FOV-resolution ratio of >3,000, while securing up-to a 1.6 mm Nyquist-satisfied aliasing-free FOV, a ∼400 nm lateral resolution, and a 70 M/s effective voxel-sampling rate, all at the same time. Competing Interests: The mesoscopic MPM is under national-phase patent applications through the Patent Cooperation Treaty (PCT), publication number: WO/2021/112942; a granted patent TWI714378B (Taiwan); inventors: C.-K. Sun & B. J. Borah; applicant: National Taiwan University, Taiwan. The compiled standalone acquisition software, being a part of the patent, will be available from the corresponding author upon reasonable request, for non-commercial use only, subject to regulation and approval from National Taiwan University, Taiwan. (© 2021 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu