Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits.

Autor: Bertens CJF; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands. christian.bertens@mumc.nl.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands. christian.bertens@mumc.nl., van Mechelen RJS; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands., Berendschot TTJM; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands., Gijs M; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands., Wolters JEJ; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands., Gorgels TGMF; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands., Nuijts RMMA; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands., Beckers HJM; Department of Ophthalmology, University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University Medical Center+ (MUMC+), P. Debyelaan 25, PO Box 5800, 6229 HX, Maastricht, The Netherlands.; Chemelot Institute for Science and Technology (InSciTe), Gaetano Martinolaan 63-65, 6229 GS, Maastricht, The Netherlands.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Sep 28; Vol. 11 (1), pp. 19217. Date of Electronic Publication: 2021 Sep 28.
DOI: 10.1038/s41598-021-98762-7
Abstrakt: The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R 2  = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.
(© 2021. The Author(s).)
Databáze: MEDLINE
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