Analysis of the nano and microstructures of the cervical cementum and saliva in periodontitis: A pilot study.

Autor: Parlak HM; Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. Electronic address: mervaparlakdt@gmail.com., Karaarslan D; Department of Physics Engineering, Hacettepe University, Ankara, Turkey., Ide S; Department of Physics Engineering, Hacettepe University, Ankara, Turkey., Çelik HH; Department of Anatomy, Hacettepe University Faculty of Medicine, Ankara, Turkey., Karabulut E; Department of Biostatistics, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Ertan AA; Department of Prosthodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey., Akalın FA; Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Journal of oral biosciences [J Oral Biosci] 2021 Dec; Vol. 63 (4), pp. 370-377. Date of Electronic Publication: 2021 Sep 25.
DOI: 10.1016/j.job.2021.09.007
Abstrakt: Objectives: During the progression of periodontitis, the structures of the cementum and saliva are altered due to pathological changes in the environment. This study aimed to analyze the nanostructures of the cervical cementum and saliva in patients with periodontitis.
Methods: Patients with periodontitis (n = 10) and periodontally healthy controls (n = 8) were included. Single-rooted teeth with indications for extraction were obtained from individuals. The cervical-thirds of the roots were sectioned transversely to obtain 1 mm thick sections. Unstimulated whole saliva samples were collected from each individual. The nanostructures of the cementum and saliva were analyzed using small and wide-angle X-ray scattering methods.
Results: The mean radius and distance values of the cementum nanoparticles in the periodontitis and control groups were 368 Å and 1152 Å, and 377 Å and 1186 Å, respectively. The mean radius and distance values of the saliva nanoparticles in the periodontitis and control groups were 425 Å and 1359 Å, and 468 Å and 1452 Å, respectively. More wide-angle X-ray scattering profile peaks were observed in the cementum of the controls. Similarities were observed between the 3D profiles of the cementum and the saliva nanoparticles.
Conclusions: According to the results of the present study, (i) the cementum and saliva nanoparticles were of similar size in periodontitis and healthy controls, (ii) the cementum was more crystalline according to the (002) crystallographic plane in controls, and (iii) the similarities in the 3D-profile of the cementum and saliva nanoparticles suggest some interactions between them in the sulcus/periodontal pocket at the nanolevel.
Competing Interests: Conflicts of interest The authors declare no conflicts of interest related to this study.
(Copyright © 2021 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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