A BioID-derived proximity interactome for SARS-CoV-2 proteins.

Autor:	May DG; Enabling Technologies Group, Sanford Research, Sioux Falls, SD., Martin-Sancho L; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA., Anschau V; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA., Liu S; Division of Genetics, Department of Medicine, University of California, San Diego, CA., Chrisopulos RJ; Enabling Technologies Group, Sanford Research, Sioux Falls, SD., Scott KL; Enabling Technologies Group, Sanford Research, Sioux Falls, SD., Halfmann CT; Enabling Technologies Group, Sanford Research, Sioux Falls, SD., Peña RD; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA., Pratt D; Division of Genetics, Department of Medicine, University of California, San Diego, CA., Campos AR; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA., Roux KJ; Enabling Technologies Group, Sanford Research, Sioux Falls, SD.; Department of Pediatrics, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2021 Sep 21. Date of Electronic Publication: 2021 Sep 21.
DOI:	10.1101/2021.09.17.460814
Abstrakt:	The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here we use BioID to map the SARS-CoV-2 virus-host interactome using human lung cancer derived A549 cells expressing individual SARS-CoV-2 viral proteins. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are in association with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Collectively, these studies provide a valuable resource to potentially uncover novel SARS-CoV-2 biology and inform development of antivirals.
Databáze:	MEDLINE
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