Bordered Pit Formation in Cell Walls of Spruce Tracheids.
| Autor: | Chukhchin DG; Department of Biology, Ecology and Biotechnology, Northern (Arctic) Federal University, Northern Dvina Embankment 17, 163002 Arkhangelsk, Russia., Vashukova K; Department of Biology, Ecology and Biotechnology, Northern (Arctic) Federal University, Northern Dvina Embankment 17, 163002 Arkhangelsk, Russia., Novozhilov E; Department of Biology, Ecology and Biotechnology, Northern (Arctic) Federal University, Northern Dvina Embankment 17, 163002 Arkhangelsk, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Plants (Basel, Switzerland) [Plants (Basel)] 2021 Sep 21; Vol. 10 (9). Date of Electronic Publication: 2021 Sep 21. |
| DOI: | 10.3390/plants10091968 |
| Abstrakt: | The process of pit formation in plants still has various questions unaddressed and unknown, which opens up many interesting and new research opportunities. The aim of this work was elucidation of the mechanism for the formation of bordered pits of the spruce ( Picea abies (L.) Karst.) tracheid with exosomes participation and mechanical deformation of the cell wall. Sample sections were prepared from spruce stem samples after cryomechanical destruction with liquid nitrogen. The study methods included scanning electron microscopy and enzymatic treatment. Enzymatic treatment of the elements of the bordered pit made it possible to clarify the localization of cellulose and pectin. SEM images of intermediate stages of bordered pit formation in the radial and tangential directions were obtained. An asynchronous mechanism of formation of bordered-pit pairs in tracheids is proposed. The formation of the pit pair begins from the side of the initiator cell and is associated with enzymatic hydrolysis of the secondary cell wall and subsequent mechanical deformation of the primary cell walls. Enzymatic hydrolysis of the S |
| Databáze: | MEDLINE |
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