Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay.

Autor: Beddingfield BJ; Department of Microbiology and Immunology, School of Medicine, Tulane University, New Orleans, LA 70112, USA., Hartnett JN; Department of Microbiology and Immunology, School of Medicine, Tulane University, New Orleans, LA 70112, USA., Wilson RB; Autoimmune Technologies, Limited Liability Company, New Orleans, LA 70112, USA., Kulakosky PC; Autoimmune Technologies, Limited Liability Company, New Orleans, LA 70112, USA., Andersen KG; Department of Immunology and Microbial Science, Scripps Research, La Jolla, CA 92037, USA.; Scripps Translational Science Institute, La Jolla, CA 92037, USA.; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA 92037, USA., Robles-Sikisaka R; Department of Immunology and Microbial Science, Scripps Research, La Jolla, CA 92037, USA.; Scripps Translational Science Institute, La Jolla, CA 92037, USA.; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA 92037, USA., Grubaugh ND; Department of Immunology and Microbial Science, Scripps Research, La Jolla, CA 92037, USA.; Scripps Translational Science Institute, La Jolla, CA 92037, USA.; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA 92037, USA., Aybar A; MediPath Instituto de Patologia Molecular, Universidad Tecnológica de Santiago (UTESA), Santiago 51000, Dominican Republic., Nunez MZ; Centro de Investigaciones Biomédicas y Clínicas (CINBIOCLI), Pontificia Universidad Católica Madre y Maestra (PUCMM), Santiago 51034, Dominican Republic., Fermin CD; Instituto de Innovacion Biotecnologia e Industria (IIBI), Santo Domingo 10135, Dominican Republic.; Ministerio de Salud Publica (MSP), Santo Domingo 10514, Dominican Republic., Garry RF; Department of Microbiology and Immunology, School of Medicine, Tulane University, New Orleans, LA 70112, USA.; Zalgen Labs, Limited Liability Company, Germantown, MD 20876, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2021 Sep 05; Vol. 13 (9). Date of Electronic Publication: 2021 Sep 05.
DOI: 10.3390/v13091771
Abstrakt: Infection with Zika virus (ZIKV), a member of the Flavivirus genus of the Flaviviridae family, typically results in mild self-limited illness, but severe neurological disease occurs in a limited subset of patients. In contrast, serious outcomes commonly occur in pregnancy that affect the developing fetus, including microcephaly and other major birth defects. The genetic similarity of ZIKV to other widespread flaviviruses, such as dengue virus (DENV), presents a challenge to the development of specific ZIKV diagnostic assays. Nonstructural protein 1 (NS1) is established for use in immunodiagnostic assays for flaviviruses. To address the cross-reactivity of ZIKV NS1 with proteins from other flaviviruses we used site-directed mutagenesis to modify putative epitopes. Goat polyclonal antibodies to variant ZIKV NS1 were affinity-purified to remove antibodies binding to the closely related NS1 protein of DENV. An antigen-capture ELISA configured with the affinity-purified polyclonal antibody showed a linear dynamic range between approximately 500 and 30 ng/mL, with a limit of detection of between 1.95 and 7.8 ng/mL. NS1 proteins from DENV, yellow fever virus, St. Louis encephalitis virus and West Nile virus showed significantly reduced reactivity in the ZIKV antigen-capture ELISA. Refinement of approaches similar to those employed here could lead to development of ZIKV-specific immunoassays suitable for use in areas where infections with related flaviviruses are common.
Databáze: MEDLINE
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