Tracing Viral Transmission and Evolution of Bovine Leukemia Virus through Long Read Oxford Nanopore Sequencing of the Proviral Genome.

Autor:	Pavliscak LA; CentralStar Cooperative, Lansing, MI 48910, USA., Nirmala J; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA., Singh VK; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA., Sporer KRB; CentralStar Cooperative, Lansing, MI 48910, USA., Taxis TM; Department of Large Animal Clinical Science, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA., Kumar P; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA., Goyal SM; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA., Mor SK; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA., Schroeder DC; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA.; School of Biological Sciences, University of Reading, Reading RG6 6AS, UK., Wells SJ; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA., Droscha CJ; CentralStar Cooperative, Lansing, MI 48910, USA.; Department of Large Animal Clinical Science, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2021 Sep 14; Vol. 10 (9). Date of Electronic Publication: 2021 Sep 14.
DOI:	10.3390/pathogens10091191
Abstrakt:	Bovine leukemia virus (BLV) causes Enzootic Bovine Leukosis (EBL), a persistent life-long disease resulting in immune dysfunction and shortened lifespan in infected cattle, severely impacting the profitability of the US dairy industry. Our group has found that 94% of dairy farms in the United States are infected with BLV with an average in-herd prevalence of 46%. This is partly due to the lack of clinical presentation during the early stages of primary infection and the elusive nature of BLV transmission. This study sought to validate a near-complete genomic sequencing approach for reliability and accuracy before determining its efficacy in characterizing the sequence identity of BLV proviral genomes collected from a pilot study made up of 14 animals from one commercial dairy herd. These BLV-infected animals were comprised of seven adult dam/daughter pairs that tested positive by ELISA and qPCR. The results demonstrate sequence identity or divergence of the BLV genome from the same samples tested in two independent laboratories, suggesting both vertical and horizontal transmission in this dairy herd. This study supports the use of Oxford Nanopore sequencing for the identification of viral SNPs that can be used for retrospective genetic contact tracing of BLV transmission.
Databáze:	MEDLINE
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