| Autor: |
Kutzer P; Landeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), Germany., Szentiks CA; Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, Germany., Bock S; Landeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), Germany., Fritsch G; Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, Germany., Magyar T; Veterinary Medical Research Institute, Eötvös Lóránd Research Network (ELKH), 1143 Budapest, Hungary., Schulze C; Landeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), Germany., Semmler T; Microbial Genomics, Robert Koch Institute, 13353 Berlin, Germany., Ewers C; Faculty of Veterinary Medicine, Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, Germany. |
| Abstrakt: |
Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella ( P .) multocida capsular type B ( Pm B) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf ( Canis lupus ) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German Pm B-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 Pm B isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three Pm B isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 Pm B-HS-isolates and the three Pm B-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122 RIRDC and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122 RIRDC strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of Pm B-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour Pm B as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany. |
