Enhanced Antiviral Function of Magnesium Chloride-Modified Heparin on a Broad Spectrum of Viruses.

Autor: Mese K; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany., Bunz O; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany.; Department of Prosthodontics, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, Germany., Volkwein W; Bavarian Health and Food Safety Authority (LGL), 85764 Oberschleissheim, Germany., Vemulapalli SPB; Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany., Zhang W; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany., Schellhorn S; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany., Heenemann K; Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, 04103 Leipzig, Germany., Rueckner A; Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, 04103 Leipzig, Germany., Sing A; Bavarian Health and Food Safety Authority (LGL), 85764 Oberschleissheim, Germany., Vahlenkamp TW; Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, 04103 Leipzig, Germany., Severing AL; Centre for Biomedical Education and Research (ZBAF), Institute for Translational Wound Research, Witten/Herdecke University, 58453 Witten, Germany., Gao J; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany., Aydin M; Center for Child and Adolescent Medicine, Center for Clinical and Translational Research (CCTR), Helios University Hospital Wuppertal, Witten/Herdecke University, 42283 Wuppertal, Germany., Jung D; Centre for Biomedical Education and Research, Institute of Pharmacology and Toxicology, Witten/Herdecke University, 58453 Witten, Germany., Bachmann HS; Centre for Biomedical Education and Research, Institute of Pharmacology and Toxicology, Witten/Herdecke University, 58453 Witten, Germany., Zänker KS; Center for Biomedical Education and Research (ZBAF), Institute of Immunology, Witten/Herdecke University, 58453 Witten, Germany., Busch U; Bavarian Health and Food Safety Authority (LGL), 85764 Oberschleissheim, Germany., Baiker A; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany.; Bavarian Health and Food Safety Authority (LGL), 85764 Oberschleissheim, Germany., Griesinger C; Centre for Biomedical Education and Research, Institute of Pharmacology and Toxicology, Witten/Herdecke University, 58453 Witten, Germany., Ehrhardt A; Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2021 Sep 17; Vol. 22 (18). Date of Electronic Publication: 2021 Sep 17.
DOI: 10.3390/ijms221810075
Abstrakt: Previous studies reported on the broad-spectrum antiviral function of heparin. Here we investigated the antiviral function of magnesium-modified heparin and found that modified heparin displayed a significantly enhanced antiviral function against human adenovirus (HAdV) in immortalized and primary cells. Nuclear magnetic resonance analyses revealed a conformational change of heparin when complexed with magnesium. To broadly explore this discovery, we tested the antiviral function of modified heparin against herpes simplex virus type 1 (HSV-1) and found that the replication of HSV-1 was even further decreased compared to aciclovir. Moreover, we investigated the antiviral effect against the new severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and measured a 55-fold decreased viral load in the supernatant of infected cells associated with a 38-fold decrease in virus growth. The advantage of our modified heparin is an increased antiviral effect compared to regular heparin.
Databáze: MEDLINE