| Autor: |
Carolus H; Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology, KU Leuven, 3001 Leuven, Belgium.; VIB-KU Leuven Center for Microbiology, 3001 Leuven, Belgium., Jacobs S; Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology, KU Leuven, 3001 Leuven, Belgium., Lobo Romero C; Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology, KU Leuven, 3001 Leuven, Belgium.; VIB-KU Leuven Center for Microbiology, 3001 Leuven, Belgium., Deparis Q; VIB-KU Leuven Center for Microbiology, 3001 Leuven, Belgium.; Laboratory for Genetics and Genomics, Centre for Microbial and Plant Genetics, KU Leuven, 3001 Leuven, Belgium., Cuomo CA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Meis JF; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, 6532 Nijmegen, The Netherlands.; Centre of Expertise in Mycology Radboudumc/CWZ, 6532 Nijmegen, The Netherlands., Van Dijck P; Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology, KU Leuven, 3001 Leuven, Belgium.; VIB-KU Leuven Center for Microbiology, 3001 Leuven, Belgium. |
| Abstrakt: |
Candida auris is an opportunistic pathogenic yeast that emerged worldwide during the past decade. This fungal pathogen poses a significant public health threat due to common multidrug resistance (MDR), alarming hospital outbreaks, and frequent misidentification. Genomic analyses have identified five distinct clades that are linked to five geographic areas of origin and characterized by differences in several phenotypic traits such as virulence and drug resistance. Typing of C. auris strains and the identification of clades can be a powerful tool in molecular epidemiology and might be of clinical importance by estimating outbreak and MDR potential. As C. auris has caused global outbreaks, including in low-income countries, typing C. auris strains quickly and inexpensively is highly valuable. We report five allele-specific polymerase chain reaction (AS-PCR) assays for the identification of C. auris and each of the five described clades of C. auris based on conserved mutations in the internal transcribed spacer (ITS) rDNA region and a clade-specific gene cluster. This PCR method provides a fast, cheap, sequencing-free diagnostic tool for the identification of C. auris , C. auris clades, and potentially, the discovery of new clades. |
