MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation.

Autor:	Frye RE; Barrow Neurological Institute at Phoenix Children's Hospital, Phoenix, AZ 85016, USA.; Department of Child Health, University of Arizona College of Medicine, Phoenix, AZ 85004, USA., Rose S; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA.; Arkansas Children's Research Institute, Little Rock, AR 72202, USA., McCullough S; Arkansas Children's Research Institute, Little Rock, AR 72202, USA., Bennuri SC; Arkansas Children's Research Institute, Little Rock, AR 72202, USA., Porter-Gill PA; Arkansas Children's Research Institute, Little Rock, AR 72202, USA., Dweep H; The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA., Gill PS; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA.; Arkansas Children's Research Institute, Little Rock, AR 72202, USA.
Jazyk:	angličtina
Zdroj:	Journal of personalized medicine [J Pers Med] 2021 Sep 17; Vol. 11 (9). Date of Electronic Publication: 2021 Sep 17.
DOI:	10.3390/jpm11090922
Abstrakt:	Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unrelated age- and gender-matched controls and 10 typically developing siblings. Small RNAseq analysis identified miRNAs, and selected miRNAs were validated using quantitative real-time polymerase reaction (qRT-PCR). KEGG analysis identified target pathways, and selected predicted mRNAs were validated using qRT-PCR. Results: Small RNAseq analysis identified that multiple miRNAs differentiated ASD from unrelated controls and ASD from typically developing siblings, with only one, hsa-miR-451a_R-1, being in common. Verification with qRT-PCR showed that miR-320a differentiated ASD from both sibling and unrelated controls and that several members of the miR-181 family differentiated ASD from unrelated controls. Differential expression of AKT2, AKT3, TNF α and CamKinase II predicted by KEGG analysis was verified by qRT-PCR. Expression of CamKinase II βwas found to be correlated with the severity of stereotyped behavior of the ASD participants. Conclusions: This study provides insight into the mechanisms regulating molecular pathways in individuals with ASD and identifies differentiated regulated genes involved in both the central nervous system and the immune system.
Databáze:	MEDLINE
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