| Autor: |
Osik NA; International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, Russia.; Physical Department, Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia., Zelentsova EA; International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, Russia.; Physical Department, Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia., Tsentalovich YP; International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, Russia.; Physical Department, Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia. |
| Abstrakt: |
Ovothiol A (OSH) is one of the strongest natural antioxidants. So far, its presence was found in tissues of marine invertebrates, algae and fish. Due to very low pKa value of the SH group, under physiological conditions, this compound is almost entirely present in chemically active thiolate form and reacts with ROS and radicals significantly faster than other natural thiols. In biological systems, OSH acts in tandem with glutathione GSH, with OSH neutralizing oxidants and GSH maintaining ovothiol in the reduced state. In the present work, we report the rate constants of OSH oxidation by H 2 O 2 and of reduction of oxidized ovothiol OSSO by GSH and we estimate the Arrhenius parameters for these rate constants. The absorption spectra of reaction intermediates, adduct OSSG and sulfenic acid OSOH, were obtained. We also found that OSH effectively quenches the triplet state of kynurenic acid with an almost diffusion-controlled rate constant. This finding indicates that OSH may serve as a good photoprotector to inhibit the deleterious effect of solar UV irradiation; this assumption explains the high concentrations of OSH in the fish lens. The unique antioxidant and photoprotecting properties of OSH open promising perspectives for its use in the treatment of human diseases. |
