Complementary Effect of Non-Persistent Silver Nano-Architectures and Chlorhexidine on Infected Wound Healing.

Autor: Pernakov M; Sumy State University, 40007 Sumy, Ukraine., Ermini ML; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, 56127 Pisa, Italy., Sulaieva O; Medical Laboratory CSD, 03148 Kyiv, Ukraine., Cassano D; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, 56127 Pisa, Italy., Santucci M; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, 56127 Pisa, Italy., Husak Y; Sumy State University, 40007 Sumy, Ukraine., Korniienko V; Sumy State University, 40007 Sumy, Ukraine., Giannone G; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, 56127 Pisa, Italy.; NEST-Scuola Normale Superiore, 56127 Pisa, Italy., Yusupova A; Sumy State University, 40007 Sumy, Ukraine., Liubchak I; Sumy State University, 40007 Sumy, Ukraine.; The University of Western Ontario, London, ON N6A 3K7, Canada., Hristova MT; Medical University of Pleven, 5800 Pleven, Bulgaria., Savchenko A; Sumy State University, 40007 Sumy, Ukraine., Holubnycha V; Sumy State University, 40007 Sumy, Ukraine., Voliani V; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, 56127 Pisa, Italy., Pogorielov M; Sumy State University, 40007 Sumy, Ukraine.; NanoPrime, 39200 Debica, Poland.
Jazyk: angličtina
Zdroj: Biomedicines [Biomedicines] 2021 Sep 14; Vol. 9 (9). Date of Electronic Publication: 2021 Sep 14.
DOI: 10.3390/biomedicines9091215
Abstrakt: Surgical site infection (SSI) substantially contributes each year to patients' morbidity and mortality, accounting for about 15% of all nosocomial infections. SSI drastically increases the rehab stint and expenses while jeopardizing health outcomes. Besides prevention, the treatment regime relies on an adequate antibiotic therapy. On the other hand, resistant bacterial strains have currently reached up to 34.3% of the total infections, and this percentage grows annually, reducing the efficacy of the common treatment schemes. Thus, new antibacterial strategies are urgently demanded. Here, we demonstrated in rats the effectiveness of non-persistent silver nano-architectures (AgNAs) in infected wound healing together with their synergistic action in combination with chlorhexidine. Besides the in vivo efficacy evaluation, we performed analysis of the bacteriological profile of purulent wound, histological evaluations, and macrophages polarization quantifications to further validate our findings and elucidate the possible mechanisms of AgNAs action on wound healing. These findings open the way for the composition of robust multifunctional nanoplatforms for the translation of safe and efficient topical treatments of SSI.
Databáze: MEDLINE