The involvement of orexin 1 and cannabinoid 1 receptors within the ventrolateral periaqueductal gray matter in the modulation of migraine-induced anxiety and social behavior deficits of rats.

Autor: Pourrahimi AM; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran., Abbasnejad M; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Raoof M; Department of Orofacial Pain and Dysfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Endodontology Research Center, Kerman University of Medical Sciences, Kerman, Iran., Esmaeili-Mahani S; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Kooshki R; Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran. Electronic address: kooshki.r@lu.ac.ir.
Jazyk: angličtina
Zdroj: Peptides [Peptides] 2021 Dec; Vol. 146, pp. 170651. Date of Electronic Publication: 2021 Sep 21.
DOI: 10.1016/j.peptides.2021.170651
Abstrakt: Orexin 1 receptors (Orx1R) and cannabinoid 1 receptors (CB1R) are implicated in migraine pathophysiology. This study evaluated the potential involvement of Orx1R and CB1R within the ventrolateral periaqueductal gray matter (vlPAG) in the modulation of anxiety-like behavior and social interaction of migraineurs rats. A rat model of migraine induced by recurrent administration of nitroglycerin (NTG) (5 mg/kg/i.p.). The groups of rats (n = 6) were then subjected to intra-vlPAG microinjection of orexin-A (25, 50 pM), and Orx1R antagonist SB334867 (20, 40 nM) or AM 251 (2, 4 μg) as a CB1R antagonist. Behavioral responses were evaluated in elevated plus maze (EPM), open field (OF) and three-chambered social test apparatus. NTG produced a marked anxiety like behaviors, in both EPM and OF tasks. It did also decrease social performance. NTG-related anxiety and social conflicts were attenuated by orexin-A (25, 50 pM). However, NTG effects were exacerbated by SB334867 (40 nM) and AM251 (2, 4 μg). The orexin-A-mediated suppression of NTG-induced anxiety and social conflicts were prevented by either SB334867 (20 nM) or AM251 (2 μg). The findings suggest roles for Orx1R and CB1R signaling within vlPAG in the modulation of migraine-induced anxiety-like behavior and social dysfunction in rats.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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