Direct coculture of human pluripotent stem cell-derived cardiac progenitor cells with epicardial cells induces cardiomyocyte proliferation and reduces sarcomere organization.

Autor: Floy ME; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Dunn KK; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Mateyka TD; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Reichardt IM; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Steinberg AB; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Palecek SP; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: sppalecek@wisc.edu.
Jazyk: angličtina
Zdroj: Journal of molecular and cellular cardiology [J Mol Cell Cardiol] 2022 Jan; Vol. 162, pp. 144-157. Date of Electronic Publication: 2021 Sep 22.
DOI: 10.1016/j.yjmcc.2021.09.009
Abstrakt: Epicardial cells (EpiCs) are necessary for myocardium formation, yet little is known about crosstalk between EpiCs and cardiomyocytes (CMs) during development and the potential impact of EpiCs on CM maturation. To investigate the effects of EpiCs on CM commitment and maturation, we differentiated human pluripotent stem cells (hPSCs) to cardiac progenitor cells (CPCs) and EpiCs, and cocultured EpiCs and CPCs for two weeks. When EpiCs were allowed to form epicardial-derived cells, we observed increased expression of cTnI in developing CMs. In the presence of the TGFβ inhibitor A83-01, EpiCs remained in the epicardial state and induced CM proliferation, increased MLC2v expression, and led to less organized sarcomeres. These effects were not observed if CPCs were treated with EpiC-conditioned medium or if CPCs were indirectly cocultured with EpiCs. Finally, single cell RNA sequencing identified that EpiC-CPC coculture had bi-directional effects on transcriptional programs in EpiCs and CMs, and biased EpiC lineages from a SFRP2-enriched population to a DLK1- or C3-enriched population. This work suggests important crosstalk between EpiCs and CMs during differentiation which can be used to influence cell fate and improve the ability to generate cardiac cells and tissues for in vitro models and development of cardiac cellular therapies.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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