Supernova: A Deoxyribozyme that Catalyzes a Chemiluminescent Reaction.

Autor: Svehlova K; Institute of Organic Chemistry and Biochemistry ASCR, Prague, Czech Republic.; Faculty of Science, Charles University in Prague, Prague, Czech Republic., Lukšan O; Institute of Organic Chemistry and Biochemistry ASCR, Prague, Czech Republic., Jakubec M; Institute of Organic Chemistry and Biochemistry ASCR, Prague, Czech Republic.; Faculty of Science, Charles University in Prague, Prague, Czech Republic., Curtis EA; Institute of Organic Chemistry and Biochemistry ASCR, Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2022 Jan 17; Vol. 61 (3), pp. e202109347. Date of Electronic Publication: 2021 Nov 25.
DOI: 10.1002/anie.202109347
Abstrakt: Functional DNA molecules are useful components in nanotechnology and synthetic biology. To expand the toolkit of functional DNA parts, in this study we used artificial evolution to identify a glowing deoxyribozyme called Supernova. This deoxyribozyme transfers a phosphate from a 1,2-dioxetane substrate to its 5' hydroxyl group, which triggers a chemiluminescent reaction and a flash of blue light. An engineered version of Supernova is only catalytically active in the presence of an oligonucleotide complementary to its 3' end, demonstrating that light production can be coupled to ligand binding. We anticipate that Supernova will be useful in a wide variety of applications, including as a signaling component in allosterically regulated sensors and in logic gates of molecular computers.
(© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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