Evaluation of Blend Production of Cellulases and Xylanases Using Pretreated and Recycled Carnauba Straw.

Autor: da Silva FL; Chemical Engineering Department, Biochemical Engineering Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.; Federal Institute of Education, Science, and Technology of Paraiba (IFPB) - Campus Picui, Picui, PB, Brazil., Dos Santos DA; Chemical Engineering Department, Biochemical Engineering Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil., de Oliveira Campos A; Chemical Engineering Department, Biochemical Engineering Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil., Magalhães ERB; Chemical Engineering Department, Biochemical Engineering Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.; Northeast Strategic Technologies Center (CETENE), Recife, PE, Brazil., Dos Santos ES; Chemical Engineering Department, Biochemical Engineering Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil. everaldo@eq.ufrn.br.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2022 Feb; Vol. 194 (2), pp. 901-913. Date of Electronic Publication: 2021 Sep 24.
DOI: 10.1007/s12010-021-03677-8
Abstrakt: Carnauba (Copernicia prunifera) is a Brazilian palm tree used for wax production, which usually generates a large amount of waste. This work evaluated the carnauba waste for cellulase and xylanase production using Trichoderma reesei CCT2768 through a solid-state fermentation (SSF). Carnauba waste was used in its crude form (C-IN), pretreated (C-P) with alkaline hydrogen peroxide (AHP), and also recycled after the SSF process (C-PR). C-IN, C-P, and C-PR were characterized by XRD, FTIR, and SEM. Cellulase and xylanase production was performed by SSF for 72 h, and the enzymatic extracts obtained were mixed each other in different concentrations. FPase, CMCase, and xylanase activities were determined. Trichoderma reesei CCT-2768 has shown high performance to produce cellulases and xylanases. Total cellulase, CMCase, and β-glycosidase presented a highest activity when C-PPR1 (25% of C-PR and 75% of C-P) was used as a carbon source, with yield of 2.85 U/g, 41.21 U/g, and 2.80 U/g, respectively. The highest xylanase production was achieved when only the pretreated carnauba waste (C-P) was used, with an enzyme activity of 224.93 U/g. Carnauba has shown a promising carbon source capacity to induce the production of cellulolytic and xylanolytic enzymes by using T. reesei CCT2768, promoting the circular and ecofriendly economy, as well as a cost reduction, of the production process of these enzymes.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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