| Autor: |
Dkhili S; Laboratoire des Microorganismes et Biomolécules actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, Tunisie.; Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, Tunis, Tunisie., Ribeiro M; Department of Genetics and Biotechnology and University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.; Functional Genomics and Proteomics Unity, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.; LAQV-REQUIMTE, Faculty of Science and Technology, University Nova of Lisbon, Lisbon, Portugal., Ghariani S; Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, Tunis, Tunisie., Yahia HB; Laboratoire des Microorganismes et Biomolécules actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, Tunisie.; Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, Tunis, Tunisie., Hillion M; University Clermont Auvergne, INRAE, UMR0454 Microbiology Digestive Environment Health (MEDiS), Saint-Genès Champanelle, France.; INRAE, Metabolism Exploration Platform, Proteomic Component (PFEMcp), Saint-Genès Champanelle, France., Poeta P; Department of Genetics and Biotechnology and University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.; Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal., Slama KB; Laboratoire des Microorganismes et Biomolécules actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, Tunisie.; Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, Tunis, Tunisie., Hébraud M; University Clermont Auvergne, INRAE, UMR0454 Microbiology Digestive Environment Health (MEDiS), Saint-Genès Champanelle, France.; INRAE, Metabolism Exploration Platform, Proteomic Component (PFEMcp), Saint-Genès Champanelle, France., Igrejas G; Department of Genetics and Biotechnology and University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.; Functional Genomics and Proteomics Unity, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.; LAQV-REQUIMTE, Faculty of Science and Technology, University Nova of Lisbon, Lisbon, Portugal. |
| Abstrakt: |
With the emergence of multiresistant bacteria, the use of bacteriophages is gaining renewed interest as potential antimicrobial agents. The aim of this study was to analyze the structure of three lytic bacteriophages infecting Escherichia coli (SD1, SD2, and SD3) using a gel-based proteomics approach and the cellular response of this bacterium to phage SD1 infection at the proteome level. The combination of the results of 1-DE and 2-DE followed by mass spectrometry led to the identification of 3, 14, and 9 structure proteins for SD1, SD2, and SD3 phages, respectively. Different protein profiles with common proteins were noticed. We also analyzed phage-induced effects by comparing samples from infected cells to those of noninfected cells. We verified important changes in E. coli proteins expression during phage SD1 infection, where there was an overexpression of proteins involved in stress response. Our results indicated that viral infection caused bacterial oxidative stress and bacterial cells response to stress was orchestrated by antioxidant defense mechanisms. This article makes an empirical scientific contribution toward the concept of bacteriophages as potential antimicrobial agents. With converging ecological threats in the 21st century, novel approaches to address the innovation gaps in antimicrobial development are more essential than ever. Further research on bacteriophages is called for in this broader context of planetary health and integrative biology. |
