Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers.

Autor: Krüttgen A; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany. Electronic address: akruettgen@ukaachen.de., Klingel H; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany., Haase G; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany., Haefner H; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany., Imöhl M; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany., Kleines M; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2021 Dec; Vol. 298, pp. 114295. Date of Electronic Publication: 2021 Sep 20.
DOI: 10.1016/j.jviromet.2021.114295
Abstrakt: Current studies focus on cellular and humoral immunity induced by novel SARS-CoV-2 vaccines. Non-responders to vaccinations are not uncommonly encountered in clinical medicine (e.g. in the field of hepatitis B). Whereas vaccine-induced humoral immunity against SARS-CoV-2 is compromised by emerging Variants of Concern (VOCs), cellular immunity against SARS-CoV-2 is emerging as resilient against VOCs. Thus commercially available test kits for diagnostic laboratories designed to evaluate cellular immune responses to SARS-CoV-2 are urgently needed. Here we evaluated the novel QuantiFERON SARS-CoV-2 assay (Qiagen) measuring INF-ɣ release induced by two spike-derived peptide pools (Ag1 and Ag2) in a cohort of health care workers vaccinated with the mRNA-1273 vaccine and confirmed humoral response. Our study indicates the usefulness of this novel assay for routine laboratories to evaluate cellular immunity against SARS-CoV-2 in response to mRNA-1273 vaccination.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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