Inside the Protein Corona: From Binding Parameters to Unstained Hard and Soft Coronas Visualization.

Autor:	Galdino FE; Brazilian Synchrotron Light Laboratory (LNLS), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970 Campinas, São Paulo, Brazil.; Institute of Chemistry, University of Campinas (UNICAMP), CP 6154, CEP 13083-970 Campinas, São Paulo,Brazil., Picco AS; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Faultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, 64 y Diag. 113, 1900 La Plata, Argentina., Capeletti LB; Brazilian Synchrotron Light Laboratory (LNLS), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970 Campinas, São Paulo, Brazil., Bettini J; Brazilian Nanotechnology National Laboratory (LNNano), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970 Campinas, São Paulo, Brazil., Cardoso MB; Brazilian Synchrotron Light Laboratory (LNLS), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970 Campinas, São Paulo, Brazil.; Institute of Chemistry, University of Campinas (UNICAMP), CP 6154, CEP 13083-970 Campinas, São Paulo,Brazil.
Jazyk:	angličtina
Zdroj:	Nano letters [Nano Lett] 2021 Oct 13; Vol. 21 (19), pp. 8250-8257. Date of Electronic Publication: 2021 Sep 23.
DOI:	10.1021/acs.nanolett.1c02416
Abstrakt:	Proteins spontaneously adsorb on nanoparticle surfaces when injected into the bloodstream. It drastically modifies the nanoparticle's fate and how they interact with organs and cells. Although this protein layer (protein corona) has been widely studied, the robustness of the most employed characterization methods and the visualization of its unstained fractions remain open questions. Here, synchrotron-based small-angle X-ray scattering was used to follow the corona formation and estimate binding parameters. At the same time, transmission electron microscopy under cryogenic conditions associated with cross-correlation image processing and energy-filtered transmission electron microscopy allowed to determine protein corona morphology and thickness together with the visualization of its unstained hard and soft fractions. The above-presented strategy shows tremendous potential for deciphering fundamental protein corona aspects and can contribute to rational medical nanoparticle engineering.
Databáze:	MEDLINE
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