Flexible electrochemical aptasensor for cortisol detection in human sweat.
| Autor: | Mugo SM; Physical Sciences Department, MacEwan University, Edmonton, AB T5J 4S2, Canada. mugos@macewaan.ca., Alberkant J; Physical Sciences Department, MacEwan University, Edmonton, AB T5J 4S2, Canada. mugos@macewaan.ca., Bernstein N; Department of Biological Sciences, MacEwan University, Edmonton, Canada., Zenkina OV; Department of Chemistry, University of Ontario Institute of Technology, Ontario, Canada. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Analytical methods : advancing methods and applications [Anal Methods] 2021 Sep 23; Vol. 13 (36), pp. 4169-4173. Date of Electronic Publication: 2021 Sep 23. |
| DOI: | 10.1039/d1ay01233a |
| Abstrakt: | This communication demonstrates an electrochemical DNA aptasensor for the detection of cortisol in human sweat. The aptasensor was fabricated via layer-by-layer assembly on stretchable polydimethylsiloxane (PDMS) coated with conductive nanoporous carbon nanotube-cellulose nanocrystals (CNC/CNT) film using a linker to a cortisol specific DNA aptamer. The flexible cortisol aptasensor had a dynamic range of 2.5-35 ng mL -1 . The aptasensor precision was determined to be 2.7% relative standard deviation (%RSD) across the concentration dynamic range. The aptasensor was determined to have a limit of detection (LOD) of ∼ 1.8 ng mL -1 . The aptasensor was demonstrated to have high selectivity to cortisol and was unresponsive to interfering species including glucose, sodium lactate, and β-estradiol. The aptasensor was successfully evaluated for the detection of cortisol in human sweat indicative of its high specificity. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|