| Autor: |
O'Toole AD; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA., Zhang J; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA., Williams LBA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA., Brown CC; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2022 Jan; Vol. 34 (1), pp. 141-145. Date of Electronic Publication: 2021 Sep 23. |
| DOI: |
10.1177/10406387211047561 |
| Abstrakt: |
Formalin-fixed, paraffin-embedded tissues from European rabbits ( Oryctolagus cuniculus ) that succumbed to rabbit hemorrhagic disease virus 2 (RHDV2; Lagovirus GI.2) during the 2019 outbreak in Washington, USA, were utilized for in situ hybridization via RNAscope (ACDBio). This detection method was both sensitive and specific, with no staining in tissues from RHDV- ( Lagovirus GI.1) and RHDV2-negative rabbits, and only slight background staining of RHDV-positive rabbits; RHDV2-positive tissues had bright-red cytoplasmic staining. Although much of the viral mRNA detection was consistent with previously described antigen detection via immunohistochemistry of the liver, lungs, and spleen, there was also significant glomerular staining in the kidneys, and endothelial staining within blood vessels of almost all organs. We validated the RNAscope technique for detection of RHDV2 mRNA in formalin-fixed, paraffin-embedded tissues, with increased sensitivity from previous techniques, and identified additional affected cell types that may contribute to the understanding of pathogenesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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