The innate immune response of self-assembling silk fibroin hydrogels.

Autor: Gorenkova N; King Fahd Medical Research Center, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia.; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk.; I.M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya street, Moscow, 119991, Russian Federation., Maitz MF; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Straße 6, 01069 Dresden, Germany., Böhme G; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk.; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Straße 6, 01069 Dresden, Germany., Alhadrami HA; King Fahd Medical Research Center, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia.; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia., Jiffri EH; King Fahd Medical Research Center, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia.; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia., Totten JD; King Fahd Medical Research Center, King Abdulaziz University, P.O. BOX 80402, Jeddah 21589, Saudi Arabia.; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk., Werner C; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Straße 6, 01069 Dresden, Germany.; Technische Universität Dresden, Center for Regenerative Therapies Dresden (CRTD), Fetscherstraße 105, 01307 Dresden, Germany., Carswell HVO; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk., Seib FP; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk.; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Straße 6, 01069 Dresden, Germany.; EPSRC Future Manufacturing Research Hub for Continuous Manufacturing and Advanced Crystallisation (CMAC), University of Strathclyde, Technology and Innovation Centre, Glasgow G1 1RD, UK.
Jazyk: angličtina
Zdroj: Biomaterials science [Biomater Sci] 2021 Oct 26; Vol. 9 (21), pp. 7194-7204. Date of Electronic Publication: 2021 Oct 26.
DOI: 10.1039/d1bm00936b
Abstrakt: Silk has a long track record of use in humans, and recent advances in silk fibroin processing have opened up new material formats. However, these new formats and their applications have subsequently created a need to ascertain their biocompatibility. Therefore, the present aim was to quantify the haemocompatibility and inflammatory response of silk fibroin hydrogels. This work demonstrated that self-assembled silk fibroin hydrogels, as one of the most clinically relevant new formats, induced very low blood coagulation and platelet activation but elevated the inflammatory response of human whole blood in vitro . In vivo bioluminescence imaging of neutrophils and macrophages showed an acute, but mild, local inflammatory response which was lower than or similar to that induced by polyethylene glycol, a benchmark material. The time-dependent local immune response in vivo was corroborated by histology, immunofluorescence and murine whole blood analyses. Overall, this study confirms that silk fibroin hydrogels induce a similar immune response to that of PEG hydrogels, while also demonstrating the power of non-invasive bioluminescence imaging for monitoring tissue responses.
Databáze: MEDLINE