The Bacterial and Fungal Microbiota of the Mexican Rubiaceae Family Medicinal Plant Bouvardia ternifolia.

Autor: Villalobos-Flores LE; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Avenida Instituto Politécnico Nacional 2508, 07360, Ciudad de México, Mexico., Espinosa-Torres SD; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Avenida Instituto Politécnico Nacional 2508, 07360, Ciudad de México, Mexico., Hernández-Quiroz F; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Avenida Instituto Politécnico Nacional 2508, 07360, Ciudad de México, Mexico., Piña-Escobedo A; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Avenida Instituto Politécnico Nacional 2508, 07360, Ciudad de México, Mexico., Cruz-Narváez Y; Laboratorio de Posgrado de Operaciones Unitarias, Escuela Superior de Ingeniería Química E Industrias Extractivas del Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, 07738, Ciudad de México, Mexico., Velázquez-Escobar F; Max Volmer Laboratorium Für Biophysikalische Chemie Technische Universität Berlin, Technische Universität Berlin, Str. des 17. Juni 135/Sekr. PC-14, 10623, Berlin, Germany., Süssmuth R; Department of Chemistry, Institut Für Chemie, Technische Universität Berlin, Sekr. TC 2, Straße des 17. Juni 124, 10623, Berlin, Germany., García-Mena J; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Avenida Instituto Politécnico Nacional 2508, 07360, Ciudad de México, Mexico. jgmena@cinvestav.mx.
Jazyk: angličtina
Zdroj: Microbial ecology [Microb Ecol] 2022 Aug; Vol. 84 (2), pp. 510-526. Date of Electronic Publication: 2021 Sep 22.
DOI: 10.1007/s00248-021-01871-z
Abstrakt: Bouvardia ternifolia is a medicinal plant considered a source of therapeutic compounds, like the antitumoral cyclohexapeptide bouvardin. It is known that large number of secondary metabolites produced by plants results from the interaction of the host and adjacent or embedded microorganisms. Using high-throughput DNA sequencing of V3-16S and V5-18S ribosomal gene libraries, we characterized the endophytic, endophytic + epiphyte bacterial, and fungal communities associated to flowers, leaves, stems, and roots, as well as the rhizosphere. The Proteobacteria (average 80.7%) and Actinobacteria (average 14.7%) were the most abundant bacterial phyla, while Leotiomycetes (average 54.8%) and Dothideomycetes (average 27.4%) were the most abundant fungal classes. Differential abundance for the bacterial endophyte group showed a predominance of Erwinia, Propionibacterium, and Microbacterium genera, while Sclerotinia, Coccomyces, and Calycina genera predominated for fungi. The predictive metagenome analysis for bacteria showed significative abundance of pathways for secondary metabolite production, while a FUNguild analysis revealed the presence of pathotroph, symbiotroph, and saprotrophs in the fungal community. Intra and inter copresence and mutual exclusion interactions were identified for bacterial and fungal kingdoms in the endophyte communities. This work provides a description of the diversity and composition of bacterial and fungal microorganisms living in flowers, leaves, stems, roots, and the rhizosphere of this medicinal plant; thus, it paves the way towards an integral understanding in the production of therapeutic metabolites.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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