The Acinetobacter baumannii disinfectant resistance protein, AmvA, is a spermidine and spermine efflux pump.

Autor: Short FL; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia.; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia., Liu Q; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Shah B; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Clift HE; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia.; Versiti Blood Research Institute, Milwaukee, WI, USA., Naidu V; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Li L; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Prity FT; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Mabbutt BC; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia., Hassan KA; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia., Paulsen IT; Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia. ian.paulsen@mq.edu.au.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2021 Sep 22; Vol. 4 (1), pp. 1114. Date of Electronic Publication: 2021 Sep 22.
DOI: 10.1038/s42003-021-02629-6
Abstrakt: Antimicrobial resistance genes, including multidrug efflux pumps, evolved long before the ubiquitous use of antimicrobials in medicine and infection control. Multidrug efflux pumps often transport metabolites, signals and host-derived molecules in addition to antibiotics or biocides. Understanding their ancestral physiological roles could inform the development of strategies to subvert their activity. In this study, we investigated the response of Acinetobacter baumannii to polyamines, a widespread, abundant class of amino acid-derived metabolites, which led us to identify long-chain polyamines as natural substrates of the disinfectant efflux pump AmvA. Loss of amvA dramatically reduced tolerance to long-chain polyamines, and these molecules induce expression of amvA through binding to its cognate regulator AmvR. A second clinically-important efflux pump, AdeABC, also contributed to polyamine tolerance. Our results suggest that the disinfectant resistance capability that allows A. baumannii to survive in hospitals may have evolutionary origins in the transport of polyamine metabolites.
(© 2021. The Author(s).)
Databáze: MEDLINE
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