Articular cartilage degeneration and bone adaptation due to lack of dystrophin in mice.

Autor: Dos Santos JF; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil., Lazzarin MC; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil., Baptista VIA; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil., Quintana HT; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil., Ribeiro DA; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil., de Oliveira F; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil. flavia.oliveira@unifesp.br.
Jazyk: angličtina
Zdroj: Journal of bone and mineral metabolism [J Bone Miner Metab] 2022 Jan; Vol. 40 (1), pp. 29-39. Date of Electronic Publication: 2021 Sep 22.
DOI: 10.1007/s00774-021-01270-x
Abstrakt: Introduction: Duchenne muscular dystrophy is caused by the absence of dystrophin. This study aimed to investigate femoral morphological characteristics of lack of dystrophin in MDX mice, considering that this model, different from DMD patient, is not influenced by corticosteroids administration and limited ambulation.
Materials and Methods: Proximal femur of male 16-week-old Control and MDX mice were submitted to histological, morphometric (volume density of articular cartilage, compact bone, trabecular bone and bone marrow; articular cartilage layers area; articular cartilage cell area), and immunohistochemistry analysis for RUNX-2, RANK-L, MMP-2, MMP-9, Caspase-3 and KI-67.
Results: MDX showed loss of linearity of articular cartilage with subchondral bone transition and elevation of this subchondral bone to the articular surface when compared with control. In addition, MDX presented morphological difference in the pantographic network of collagen fibers. Volume density of trabecular bone tissue was higher in the MDX than Control, but volume density of articular cartilage was lower in MDX (p < 0.05). The articular cartilage layers and chondrocytes area were significantly smaller in MDX than Control. These results associated to MMPs and osteogenic markers of proximal femur revealed an adaptation process as a consequence of lack of dystrophin.
Conclusions: The morphological changes observed in the bone tissue of the MDX may be not only secondary to muscle weakness or chronic use of corticosteroids but also our results indicate connections between decrease of cartilage thickness, collagen network alteration and consequent subchondral changes that may lead to articular cartilage degeneration and bone adaptation mechanism in MDX mice.
(© 2021. The Japanese Society Bone and Mineral Research.)
Databáze: MEDLINE
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