Imaging in focus: An introduction to denoising bioimages in the era of deep learning.

Autor: Laine RF; MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK; The Francis Crick Institute, London NW1 1AT, UK. Electronic address: r.laine@ucl.ac.uk., Jacquemet G; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland; Åbo Akademi University, Faculty of Science and Engineering, Biosciences, 20520 Turku, Finland; Turku Bioimaging, University of Turku and Åbo Akademi University, 20520 Turku, Finland., Krull A; School of Computer Science, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. Electronic address: a.f.f.krull@bham.ac.uk.
Jazyk: angličtina
Zdroj: The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2021 Nov; Vol. 140, pp. 106077. Date of Electronic Publication: 2021 Sep 20.
DOI: 10.1016/j.biocel.2021.106077
Abstrakt: Fluorescence microscopy enables the direct observation of previously hidden dynamic processes of life, allowing profound insights into mechanisms of health and disease. However, imaging of live samples is fundamentally limited by the toxicity of the illuminating light and images are often acquired using low light conditions. As a consequence, images can become very noisy which severely complicates their interpretation. In recent years, deep learning (DL) has emerged as a very successful approach to remove this noise while retaining the useful signal. Unlike classical algorithms which use well-defined mathematical functions to remove noise, DL methods learn to denoise from example data, providing a powerful content-aware approach. In this review, we first describe the different types of noise that typically corrupt fluorescence microscopy images and introduce the denoising task. We then present the main DL-based denoising methods and their relative advantages and disadvantages. We aim to provide insights into how DL-based denoising methods operate and help users choose the most appropriate tools for their applications.
(Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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