Mitochondrial Calcium uniporters are essential for meiotic progression in mouse oocytes by controlling Ca 2+ entry.

Autor: Zhang LY; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China., Lin M; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China., Qingrui Z; Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, China Agricultural University, Beijing, China., Zichuan W; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China., Junjin L; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China., Kexiong L; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China., Xiangwei F; Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, China Agricultural University, Beijing, China.; State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China., Yunpeng H; State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Jazyk: angličtina
Zdroj: Cell proliferation [Cell Prolif] 2021 Nov; Vol. 54 (11), pp. e13127. Date of Electronic Publication: 2021 Sep 21.
DOI: 10.1111/cpr.13127
Abstrakt: Objectives: The alteration of bioenergetics by oocytes in response to the demands of various biological processes plays a critical role in maintaining normal cellular physiology. However, little is known about the association between energy sensing and energy production with energy-dependent cellular processes like meiosis.
Materials and Methods: We demonstrated that cell cycle-dependent mitochondrial Ca 2+ connects energy sensing to mitochondrial activity in meiosis progression within mouse oocytes. Further, we established a model in mouse oocytes using siRNA knockdowns that target mitochondrial calcium uniporters (MCUs) in order to inhibit mitochondrial Ca 2+ concentrations.
Results: Decreased numbers of oocytes successfully progressed to the germinal vesicle stage and extruded the first polar body during in vitro culture after inhibition, while spindle checkpoint-dependent meiosis was also delayed. Mitochondrial Ca 2+ levels changed, and this was followed by altered mitochondrial masses and ATP levels within oocytes during the entirety of meiosis progression. Abnormal mitochondrial Ca 2+ concentrations in oocytes then hindered meiotic progress and activated AMP-activated protein kinase (AMPK) signalling that is associated with gene expression.
Conclusions: These data provide new insight into the protective role that MCU-dependent mitochondrial Ca 2+ signalling plays in meiotic progress, in addition to demonstrating a new mechanism of mitochondrial energy regulation by AMPK signalling that influences meiotic maturation.
(© 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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