DARPP-32 distinguishes a subset of adult-born neurons in zebra finch HVC.

Autor: Aronowitz JV; Department of Biology, Wesleyan University, Middletown, Connecticut, USA., Kirn JR; Department of Biology, Wesleyan University, Middletown, Connecticut, USA.; Program in Neuroscience and Behavior, Wesleyan University, Middletown, Connecticut, USA., Pytte CL; Department of Psychology, Queens College and The Graduate Center, City University of New York, Flushing, New York, USA., Aaron GB; Department of Biology, Wesleyan University, Middletown, Connecticut, USA.; Program in Neuroscience and Behavior, Wesleyan University, Middletown, Connecticut, USA.
Jazyk: angličtina
Zdroj: The Journal of comparative neurology [J Comp Neurol] 2022 Apr; Vol. 530 (5), pp. 792-803. Date of Electronic Publication: 2021 Nov 15.
DOI: 10.1002/cne.25245
Abstrakt: Adult male zebra finches (Taeniopygia guttata) continually incorporate adult-born neurons into HVC, a telencephalic brain region necessary for the production of learned song. These neurons express activity-dependent immediate early genes (e.g., zenk and c-fos) following song production, suggesting that these neurons are active during song production. Half of these adult-born HVC neurons (HVC NNs) can be backfilled from the robust nucleus of the arcopallium (RA) and are a part of the vocal motor pathway underlying learned song production, but the other half do not backfill from RA, and they remain to be characterized. Here, we used cell birth-dating, retrograde tract tracing, and immunofluorescence to demonstrate that half of all HVC NNs express the phosphoprotein DARPP-32, a protein associated with dopamine receptor expression. We also demonstrate that DARPP-32+ HVC NNs are contacted by tyrosine hydroxylase immunoreactive fibers, suggesting that they receive catecholaminergic input, have transiently larger nuclei than DARPP-32-neg HVC NNs, and do not backfill from RA. Taken together, these findings help characterize a group of HVC NNs that have no apparent projections to RA and so far have eluded positive identification other than HVC NN status.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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