Analysis of the Plant Mitochondrial Transcriptome.
| Autor: | Baudry K; Institute of Plant Sciences Paris-Saclay (IPS2), Orsay, France., Delannoy E; Institute of Plant Sciences Paris-Saclay (IPS2), Orsay, France., Colas des Francs-Small C; ARC Centre of Excellence for Plant Energy Biology, The University of Western Australia, WA Crawley, Australia. catherine.colasdesfrancs-small@uwa.edu.au. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2363, pp. 235-262. |
| DOI: | 10.1007/978-1-0716-1653-6_17 |
| Abstrakt: | Transcriptome analyses are widely performed, but the analysis of organelle genome expression is often overlooked. In this chapter, we describe three methods to analyse the accumulation, splicing, editing and processing of plant mitochondrial transcript expression: a classical RT-qPCR assay, an RNA-Seq approach with its bioinformatical and statistical analysis pipeline, as well as a useful complementary technique, the Northern-blot analysis, using short biotinylated oligonucleotides as probes. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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