Osteogenic Commitment of MSC Is Enhanced after Interaction with Umbilical Cord Blood Mononuclear Cells In Vitro.

Autor: Andreeva ER; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia., Ezdakova MI; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia., Bobyleva PI; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. blastoblast@gmail.com., Andrianova IV; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia., Ratushnyy AY; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia., Buravkova LB; State Scientific Centre - Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Aug; Vol. 171 (4), pp. 541-546. Date of Electronic Publication: 2021 Sep 20.
DOI: 10.1007/s10517-021-05266-5
Abstrakt: The effectiveness of stroma-dependent expansion of hematopoietic cells ex vivo may depend on the level of commitment of multipotent mesenchymal stromal cells (MSC). Markers of MSC osteodifferentiation and the level of soluble hematopoiesis regulators were determined during their interaction with umbilical cord blood mononuclears. After 72-h co-culturing, an increase in the expression of ALPL and alkaline phosphatase activity was revealed. In conditioned medium of co-cultures, the levels of osteopontin and osteoprotegerin were elevated and the levels of osteocalcin and sclerostin were reduced. Co-culturing of umbilical cord blood mononuclears with osteocommitted MSC was accompanied by more pronounced increase in the concentration of both positive (GM-CSF and G-CSF) and negative (IP-10, MIP-1α, and MCP-3) regulators of hematopoiesis. Thus, umbilical cord blood mononuclears induced the formation of early osteogenic progenitor phenotype in MSC ex vivo, providing the microenvironmental conditions necessary to support hematopoiesis. Preliminary osteocommitted MSC were more sensitive to the effect of umbilical cord blood mononuclears.
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Databáze: MEDLINE
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